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Lecturer(s)
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Angel Roey, MSc. Dr. rer. nat.
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Bárta Jiří, doc. Ing. Ph.D.
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Kasalický Vojtěch, Mgr. Ph.D.
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Mastný Jiří, Mgr. Ph.D.
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Choma Michal, RNDr. Ph.D.
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Hájek Jan, Ph.D.
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Villena-Alemany Cristian, MSc.
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Course content
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Contents of lectures: 1. Introduction to Microbiology - importance of microorganisms - evolution of the cell (J. Szostak theory) - main microbial habitats 2. History of Microbiology - important microbiologists and discoveries 3. Seeing the small world - basic and advanced microscopical techniques, sequencing techniques 4. Cell structure - difference in prokayrotic and eukaryotic cell - inner cell structures - cytoplasmic membrane, cell wall, transport systems, DNA, basic of genetics - cytoskeleton (prokaryotic vs eukaryotic) - mitochondria, chloroplast (endo-symbiotic theory) 5. Metabolism - basic principles of metabolism and cell energetics - advanced metabolism (anaerobic respiration, methanogenesis, syntrophy, chemolithotrophy) 6. Microbial diversity - ecologically important groups of bacteria, archaea and eukaryotes - this part might be good to merge with metabolism? - advanced lecture on microbial habitats (aquatic, terestrial, higher organisms) 7. Microorganisms and their resistance to chemical agens (mainly antibiotics) - type of resistance - type of antibiotics and their targets in microbial cell (ribosome, cell wall, etc.) - fungal antibiotics 8. Biotechnology - bioremediation, biodegradation Contents of laboratories: Practical training of microscopical techniques, gram staining, streak-plate method, counting live and total bacteria, growth curve, antibiotic test.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Laboratory
- Preparation for exam
- 20 hours per semester
- Preparation for credit
- 20 hours per semester
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Learning outcomes
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The structure and functioning of microbial communities are review. An extensive introduction into field of microbiology is given.
knowledge of functioning of microbial commuities understanding of the importnce of microorganisms on our planet understanding of metabolism of microorganisms knowledge of inner structure and functioning of prokaryotic and eukaryotic cells
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Prerequisites
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basic knowledge of biochemistry and chemistry basic knowledge of laboratory work
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Assessment methods and criteria
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Oral examination, Development of laboratory protocols
Mastery of the basic course material to pass the exam (minimum 60% correct answers), preparation of laboratory protocols
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Recommended literature
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Madigan, Martinko, Dunlap, Clark. Brock:Biology of Microorganisms, 12th edition.
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