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Lecturer(s)
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Robovský Jan, RNDr. Ph.D.
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Kamiš Jan, RNDr.
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Konečná Marie, Mgr.
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Piálková Radka, RNDr. Ph.D.
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Kvičerová Jana, MVDr. Ph.D.
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Kaštovský Jan, prof. RNDr. Ph.D.
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Koutecký Petr, Mgr. Ph.D.
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Manukjanová Alžběta, Mgr. Ph.D.
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Švancárová Tereza, Mgr.
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Uhrová Michaela, Mgr.
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Šmilauerová Marie, doc. RNDr. Ph.D.
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Duda Pavel, RNDr. Ph.D.
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Course content
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Content of practices: Microcopy Theory - Physical foundations of microscopy, types of microscopes. Light microscope construction, microscopy procedure. Properties of microscopic objects, microscopic preparations. Documentation of microscopic objects. Measuring and counting microscopic objects. Contrast enhancement methods. The tasks are focused on practicing the material discussed (working with a stereomicroscope and a classical microscope, adjusting Koehler lighting, documentation, simple staining, microscopy with immersion, comparison of plant and animal tissues). Molecular biology Polymerase chain reaction and electrophoresis Phylogenetic analysis Basics of working with DNA sequences, alignment, interpretation of the phylogenetic tree
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Monitoring, Skills training, Laboratory
- Class attendance
- 36 hours per semester
- Preparation for credit
- 5 hours per semester
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Learning outcomes
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The aim of the course is to learn the basics of working in biological and molecular biological laboratories, to handle stereomicroscopes and microscopes, to learn the basics of working with the DNA sequences. The student will be able to use these skills in the following specialized courses.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Combined exam
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Recommended literature
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Habrová V. Biologická technika. 1981.
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Lacey AJ. Light Microscopy in Biology, A Practical Approach.. Oxford University Press, 1999.
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Ruzin S.E. Plant microtechnique and microscopy. Oxford University Press, 1999.
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Slonim D. Optický mikroskop, základní údaje. 2006.
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Zima J., et al. Genetické metody v zoologii. Praha, 2004.
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