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Lecturer(s)
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Juráň Josef, Mgr. Ph.D.
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Kubín Jaroslav, Mgr.
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Course content
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Content of lectures: 1. History of culturing algae, world collections of microorganisms - historically review from basic methods in cultivation of microorganisms, a major personalities and their impact; list of world collections, laws and patents. 2. Sterile work - principles of sterilization of material and media, rules for aseptic work in laminar boxes. 3. Culturing media - review of basic freshwater and marine media, alternative methods of media preparation (using natural water from localities, biphasic cultures etc.), micronutrients and buffers use. 4. Traditional methods for isolation of microalgae - enriched cultures, isolation of cell using micro-capillary, dilution techniques on agar and liquid media 5. Modern - automatized - methods of isolation of microalgae - Flow Cytometry Cell Sorting etc. 6. Methods of isolation for macroaglae - methods of purification and isolation of whole macroscopic algae thalli. 7. Maintaining of cultures - conditions and their modifications etc. 8. Cryopreservation of algae and cyanobacteria - cryoprotectants, standard and alternative methods, advantages and disadvantages. 9. Purification of strains - ways of purification of algal strains (antibiotics, inorganic compounds, mechanical procedures?) 10. Physiological aspects of algal cultivation, cells counting and grow measurement - using of algae and cyanobacteria in physiological and eco-physiological studies, experiments design (e.g. cross-gradients); counting with using light microscope, flow cytometry 11. Mass cultivations of algae and cyanobacteria - pond systems, photo-bioreactors, maricultures 12. Collections in ex-situ conservation of threatened species - case studies of using collections in practical conservation of threatened species Content of practicals: " reparation of culture media, aseptically work rules " isolation of material from natural samples by traditionally methods " preparation of material for cryopreservation " excursion to Institute of Microbiology - mass cultivations in bioreactores and Algamo company - controled cultivations
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Laboratory, Excursion
- Field trip
- 8 hours per semester
- Class attendance
- 52 hours per semester
- Preparation for credit
- 25 hours per semester
- Preparation for exam
- 40 hours per semester
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Learning outcomes
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The aim of this course is to provide a broad spectrum of information about the processing of natural material, especially isolation of microorganisms into clonal cultures. During the theoretical lectures will be presented most of common used methods of isolation, cultivation and maintaining the cultures of cyanobacteria and algae. In addition to these themes will be reflected physiological studies in cultures and methods for cryopreservation and usage of collections of algae in ex-situ conservation of endangered species. After the course, students should be able to seamlessly create a collection of algae for their research during their studies. Together with the ability to solve problems with the maintenance and cultivation of algae. In practical exercises students will learn the basics of sterile work, media preparation, isolation and purification methods.
Students will learn basic techniques of cultivation of phototrophic microorganisms.
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Prerequisites
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Knowledge of basal biological and chemical laboratory techniques.
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Assessment methods and criteria
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Student performance assessment, Test
Active participation in practical exercises is a condition of getting credit from practical part (75% attendance is minimum), passing a written test at least 50%.
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Recommended literature
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Andersen, Robert A., ed. Algal culturing techniques. Academic press, 2005..
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Becker, E. Wolfgang. Microalgae: biotechnology and microbiology. Vol. 10. Cambridge University Press, 1994..
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Janet, R., Ed Stein, and Janet R. Stein, eds. Handbook of Phycological Methods: Culture Methods and Growth Measurements. Cambridge University Press, 1973..
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Pringsheim, Ernst Georg. Algenreinkulturen: ihre Herstellung und Erhaltung. Jena: G. Fischer, 1954..
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Richmond, Amos, ed. Handbook of microalgal culture: biotechnology and applied phycology. John Wiley & Sons, 2008..
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