|
Lecturer(s)
|
-
Dadáková Eva, doc. Ing. Ph.D.
-
Bittnerová Petra, Ing. Ph.D.
|
|
Course content
|
Lectures: 1. Subject-matter of biochemistry, biochemical function and structure of a cell 2. Enzymology (structure and function of enzymes, classification of enzymes) 3. Enzymology (enzyme activity, enzyme kinetics, inhibition and activation of enzymes) 4. Biochemical energetics, metabolic pathways, regulation of enzyme reactions 5. Glycolysis, gluconeogenesis, fermentation 6. Decarboxylation of pyruvate, citric acid cycle, oxidative phosphorylation 7. Pentose cycles, metabolism of polysaccharides (glycogen) 8. Photosynthesis (light stage, dark stage C3 and C4) 9. Basic metabolism of amino-acids, ornithine cycle 11. Metabolism of nucleic acids (structure and function of nucleic acids, replication). 12. Metabolism of nucleic acids (transcription, translation). Laboratory exercises: 1. Determination of saccharides in biological material 2. Photometric determination of enzyme activity 3. Effect of enzyme concentration on reaction speed 4. Isolation of nucleic acids 5. Determination of enzyme pH-optimum 6. Effect of substrate concentration on reaction speed
|
|
Learning activities and teaching methods
|
Monologic (reading, lecture, briefing)
- Class attendance
- 28 hours per semester
- Preparation for exam
- 122 hours per semester
|
|
Learning outcomes
|
The course is focused on the introduction of the structure and properties of biopolymers and the basic principles of biochemistry of living organisms. The course introduces the basic catabolic and anabolic processes in living matter. Attention is also paid to the biochemistry of nutrition.
Based on the acquired knowledge of biochemistry, the graduate will gain the competence to use their knowledge in other chemical and scientific disciplines.
|
|
Prerequisites
|
Basic knowledge of high school biochemistry.
|
|
Assessment methods and criteria
|
Combined exam
Mastering the knowledge base of selected chapters in biochemistry about the necessary input knowledge in the field of general, inorganic, and organic chemistry, which are necessary for understanding biochemistry. Students will be acquainted with all the requirements that are mandatory for passing the course at the beginning of the semester.
|
|
Recommended literature
|
-
ŠPIČKA J.:. Biochemie, skriptum ZF JU Č.Budějovice 2004..
-
Špička,J. Biochemie. skriptum JU, 2004. ISBN 80-7040-683-6.
-
Vodrážka,Z. Biochemie, Academia Praha, 2007. 2007.
-
Vodrážka,Z. Biochemie. Academia, 2007. ISBN 978-80-200-0600-4.
-
Voet,D.-Voetová,J.G.:. Biochemie, Victoria publishing Praha 1995., 1325s..
|