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Lecturer(s)
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Čurn Vladislav, prof. Ing. Ph.D.
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Beran Pavel, Ing. Ph.D.
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Course content
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Restriction endonucleases, mapping of DNA. Bacteria in genetic engineering, plasmids, bacteriophages, cosmids, Gene libraries. Reverse transcription, cDNA library, searching for specific genes. DNA sequencing. Nucleic acids hybridisation. Structure of plant genome. Structure of genes for rRNA, tRNA, genes coding histones. Structure of genes coding proteins. Regulation of plant genome. RFLP technique in research and plant breeding. Chloroplast and mitochondrial genomes. Ti plasmids of Agrobacterium tumefaciens and Ri plasmids A.rhizogenes. Integration of T-DNA into plant genome. Genes of T-DNA region. Incorporation foreign genes into plant using A.tumefaciens. Methods of direct incorporation genes into plants. Protoplast transformation, electroporation, microinjections, microprojectile bombardment. Genetic engineering of plant viruses. Viruses as vectors for transformation. Somaclonal variation and its molecular bases. Transgenes and cultivation of GMO crops. Legal, social and ecological aspects of genetic engineering. Detection and monitoring of GMOs. Restriction endonucleases, mapping of DNA. Bacteria in genetic engineering, plasmids, bacteriophages, cosmids, Gene libraries. Reverse transcription, cDNA library, searching for specific genes. DNA sequencing. Nucleic acids hybridisation. Structure of plant genome. Structure of genes for rRNA, tRNA, genes coding histones. Structure of genes coding proteins. Regulation of plant genome. RFLP technique in research and plant breeding. Chloroplast and mitochondrial genomes. Ti plasmids of Agrobacterium tumefaciens and Ri plasmids A.rhizogenes. Integration of T-DNA into plant genome. Genes of T-DNA region. Incorporation foreign genes into plant using A.tumefaciens. Methods of direct incorporation genes into plants. Protoplast transformation, electroporation, microinjections, microprojectile bombardment. Genetic engineering of plant viruses. Viruses as vectors for transformation. Somaclonal variation and its molecular bases. Transgenes and cultivation of GMO crops. Legal, social and ecological aspects of genetic engineering. Detection and monitoring of GMOs.
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Learning activities and teaching methods
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Written action (comprehensive tests, clauses)
- Preparation for credit
- 30 hours per semester
- Preparation for exam
- 40 hours per semester
- Semestral paper
- 40 hours per semester
- Class attendance
- 56 hours per semester
- Preparation for classes
- 15 hours per semester
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Learning outcomes
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Introduction to methods of plant genetic engineering - recombinant DNA technology and transformation of plant cell. Breeding of GMO crops.
Students are able to orientate in the field of plant genetic engineering, recombinant DNA technology and methods for manipulation with plant genome
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Prerequisites
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Standard knowledge of molecular biology
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Assessment methods and criteria
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Oral examination
Active participation in exercises, protocols from laboratory exercises, understanding the curriculum defined for the exam.
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Recommended literature
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Ondřej,M. Genové inženýrství kulturních rostlin. Praha,Academia, 1992.
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Ondřej,M.,Drobník,J. Transgenoze rostlin. Academia Praha, 2002.
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Sambrook,J. a kol. Molecular cloning. New York, CSHL Press, 2001.
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