Course: Molecular Biotechnology

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Course title Molecular Biotechnology
Course code KGZB/MBI
Organizational form of instruction Lecture + Lesson + Seminary
Level of course Master
Year of study 2
Semester Summer
Number of ECTS credits 5
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Beran Pavel, Ing. Ph.D.
Course content
1.-2.Organisms used in microbial biotechnologies: Viruses, bacteria, yeasts, fungi. 3. Laboratory and industrial biotechnology. 4. Relationship between chemical production and biotechnologies. 5. Gene transfers using recombinant DNA technology. 6. Influencing gene expression in prokaryotes. Gene expression from strong and regulatable promoters. 7. Gene modification. Fusion proteins. Increasing the stability of proteins. 8. Yeast expression systems. 9. Systems using insect cells, mammalian expression systems. 10. Microbial insecticides. 11. Production of therapeutic proteins and vaccines. Gene therapy. 12. Algal biotechnology. 13.-14. Excursion to industrial operation - production of antibiotics, algal biotechnology.

Learning activities and teaching methods
Monologic (reading, lecture, briefing)
  • Preparation for credit - 25 hours per semester
  • Preparation for exam - 30 hours per semester
  • Preparation for classes - 28 hours per semester
  • Class attendance - 56 hours per semester
  • Field trip - 12 hours per semester
Learning outcomes
The course focuses on molecular biotechnology providing an overview of basic molecular biological processes in biotechnology and presenting the use of molecular biological methods for the analysis of biotechnological products. Emphasis is placed primarily on the molecular biotechnology of microorganisms.
Students are familiar with the principles of biotechnological production in small and medium scale.
Knowledge of the basics of genetics, molecular biology and basic molecular biological methods is assumed.

Assessment methods and criteria
Combined exam

This course has no prerequisites.
Recommended literature
  • David, P. Clark;. Nanette Jean Pazdernik. Biotechnology: applying the genetic revolution. Academic Press/Elsevier, 2009.
  • Glick, B. R. Molecular Biotechnology, American Society for Microbiology, 2017..
  • Šilhánková, L. Mikrobiologie pro potravináře a biotechnology. Academia, 2009.
  • Wink, M. Introducing to Molecular Biotechnology, Wiley-VCH Verlag GmbH, 2011.

Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester