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Lecturer(s)
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Beran Pavel, Ing. Ph.D.
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Stehlíková Dagmar, Ing. Ph.D.
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Course content
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1. Methods of nucleic acid extraction. 2. PCR method and nucleic acid modification. 3. Real time PCR, RT-PCR. 4. Basic methods of DNA sequencing. 5. Next generation sequencing (NGS) - Sequencing by synthesis (Illumina). 6. NGS - Pyrosequencing (454). 7. NGS - Ion semiconductor sequencing (Ion Torrent). 8. NGS - Single-molecule real-time sequencing (Pacific Biosciences). 9. NGS - Sequencing using nanopores (Oxford Nanopore). 10. RNA-seq. 11. Methods of DNA / RNA hybridization. 12. Microarray techniques. 13. Molecular cloning. 14. Bioinformatics analysis.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing)
- Preparation for credit
- 30 hours per semester
- Preparation for exam
- 30 hours per semester
- Preparation for classes
- 30 hours per semester
- Class attendance
- 56 hours per semester
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Learning outcomes
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The course deals with methods used in genetic engineering, especially in terms of nucleic acid research. It provides a theoretical and practical basis for genetic engineering of plants or animals.
Students are familiar with the methods of molecular biology and can evaluate the suitability of their application.
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Prerequisites
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Knowledge of the basics of genetics and molecular biology is assumed.
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Assessment methods and criteria
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Combined exam
Active participation in exercises, protocols from laboratory exercises, understanding the curriculum defined for the exam.
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Recommended literature
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Brown, T.A. Klonování genů a analýza DNA. UP Olomouc, 2007..
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Čurn,V. Metody GI. Č.Budějovice,ZF JU, 2005.
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Kempken, F., Jung, Ch.. Geentick modification of plants: agriculture, horticulture and forestry, Berlin, Srpinger, 2010..
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Ondřej, M., Drobník, J. Tranzgenoze rostlin, Academia Praha, 2002, 316s..
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Reece, R.J. Analysis of genes and genomes. Wiley Publishing, 2004.
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Šmarda, J. a kol. Metody molekulární biologie. 1. vyd. Brno: Masarykova univerzita, 2005.
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