Course: Methods of Genetic Engineering

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Course title Methods of Genetic Engineering
Course code KGZB/MGEI
Organizational form of instruction Lecture + Lesson
Level of course Master
Year of study 2
Semester Winter
Number of ECTS credits 5
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Beran Pavel, Ing. Ph.D.
Course content
1. Methods of nucleic acid extraction. 2. PCR method and nucleic acid modification. 3. Real time PCR, RT-PCR. 4. Basic methods of DNA sequencing. 5. Next generation sequencing (NGS) - Sequencing by synthesis (Illumina). 6. NGS - Pyrosequencing (454). 7. NGS - Ion semiconductor sequencing (Ion Torrent). 8. NGS - Single-molecule real-time sequencing (Pacific Biosciences). 9. NGS - Sequencing using nanopores (Oxford Nanopore). 10. RNA-seq. 11. Methods of DNA / RNA hybridization. 12. Microarray techniques. 13. Molecular cloning. 14. Bioinformatics analysis.

Learning activities and teaching methods
Monologic (reading, lecture, briefing)
  • Preparation for credit - 30 hours per semester
  • Preparation for exam - 30 hours per semester
  • Preparation for classes - 30 hours per semester
  • Class attendance - 56 hours per semester
Learning outcomes
The course deals with methods used in genetic engineering, especially in terms of nucleic acid research. It provides a theoretical and practical basis for genetic engineering of plants or animals.
Students are familiar with the methods of molecular biology and can evaluate the suitability of their application.
Knowledge of the basics of genetics and molecular biology is assumed.

Assessment methods and criteria
Combined exam

This course has no prerequisites.
Recommended literature
  • Brown, T.A. Klonování genů a analýza DNA. UP Olomouc, 2007..
  • Čurn,V. Metody GI. Č.Budějovice,ZF JU, 2005.
  • Kempken, F., Jung, Ch.. Geentick modification of plants: agriculture, horticulture and forestry, Berlin, Srpinger, 2010..
  • Ondřej, M., Drobník, J. Tranzgenoze rostlin, Academia Praha, 2002, 316s..
  • Reece, R.J. Analysis of genes and genomes. Wiley Publishing, 2004.
  • Šmarda, J. a kol. Metody molekulární biologie. 1. vyd. Brno: Masarykova univerzita, 2005.

Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester