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Lecturer(s)
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Čurn Vladislav, prof. Ing. Ph.D.
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Stehlíková Dagmar, Ing. Ph.D.
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Course content
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1.-2. Overview of molecular biological methods, separation techniques. 3.-5. Characteristics and overview of molecular markers, protein, isoenzyme, DNA markers, techniques based on hybridization, PCR with random and specific primers, sequencing. 6.-7. Methods of molecular data analysis. Primer design. Methods of laboratory testing of primers. 8.-9. DNA sequencing. DNA sequence analysis. 10. Statistical analysis of molecular data. 11.-12. Analysis of dominant and codominant markers. 13.-14. Use of molecular markers in genetics, breeding, systematics and population biology.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing)
- Preparation for exam
- 50 hours per semester
- Semestral paper
- 60 hours per semester
- Preparation for credit
- 30 hours per semester
- Class attendance
- 56 hours per semester
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Learning outcomes
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The aim of the course is to acquaint students with basic types of markers used in the study of income. plant populations. The subject deals with the basic distribution of markers, their description, their practical use, evaluation method and statistical evaluation.
Students are able to orientate in the field of molecular markers and their application in plant genetics and breeding
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Prerequisites
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Standard knowledge of molecular biology
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Assessment methods and criteria
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Combined exam
Active participation in exercises, protocols from laboratory exercises, understanding the curriculum defined for the exam.
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Recommended literature
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Avise, J. C. Molecular Markers, Natural History, and Evolution, Sinauer Ass., Massachusetts, 2004.
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Srivastava, S. Plant Biotechnology and Molecular Markers, Springer, 2013..
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Sucher, N.J. Plant DNA fingerprinting and Barcoding, Springer, 2012.
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Weising, K. et al. DNA fingerprinting in plants. Principles, methods, and applications. 2nd edition, 2005.
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