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Lecturer(s)
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Beran Pavel, Ing. Ph.D.
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Jozová Eva, Ing. Ph.D.
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Stehlíková Dagmar, Ing. Ph.D.
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Course content
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1.-3. - Methods of DNA isolation 4.-6. - Principle of endpoint PCR + gel electrophoresis 7.-9. - Basic overview of PCR modifications 10.-11. - Principle of DNA sequencing by Sanger method 12.-13. - Optimization of reaction 14. - Evaluation of DNA sequences
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing)
- Class attendance
- 42 hours per semester
- Preparation for classes
- 28 hours per semester
- Preparation for credit
- 20 hours per semester
- Preparation for exam
- 30 hours per semester
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Learning outcomes
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The aim of the course is to present students with basic techniques in molecular biology (especially PCR and DNA sequencing by the Sanger method) with emphasis on basic practical skills in the molecular laboratory.
Students will be able to orient in the field of laboratory techniques of molecular biology
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Prerequisites
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Standard knowledge of genetics and molecular biology
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Assessment methods and criteria
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Combined exam
Active participation in laboratora training, understanding the curriculum defined for the exam.
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Recommended literature
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Durbin, R., Eddy, S. R., Krogh, A., Mitchison, G. Biological sequence analysis. Cambridge University Press 1998, 366 s. (vybrané kapitoly).
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McPherson, M. J., Moller, S. G.. PCR The Basics, Second edition. Taylor & Francis Group 2006, 305 s.. 2006.
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Šmarda, J., Doškař, J., Pantůček, R., Růžičková, V. Metody molekulární biologie. Vydavatelství Masarykovy univerzity v Brně 2010, 188 s.. MU Brno, 2010.
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