Course: Resistance and Resistance Breeding

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Course title Resistance and Resistance Breeding
Course code KGZB/RRS
Organizational form of instruction Lecture + Lesson
Level of course Master
Year of study 1
Semester Summer
Number of ECTS credits 5
Language of instruction Czech
Status of course Compulsory-optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Jozová Eva, Ing. Ph.D.
  • Čurn Vladislav, prof. Ing. Ph.D.
Course content
Contents of blocks and seminars: 1. Genetics of resistance and breeding for resistance to abiotic and biotic stresses. 2. Definition of the resistance, classification of resistance, types of resistance. Types of hereditary resistance, oligogenic and polygenic resistance. Vertical and horizontal resistance, specific and non-specific resistance. Immunity and hypersensitivity. Field resistance. Pseudoresistance, tolerance. 3. Objectives of resistant breeding. Identification of donors and resistance genes. 4. Methods and strategies in resistant breeding. 5. Methods of resistant breeding. Breeding for oligogenic and polygenic resistance. 6. Combination crossing. 7. Backcrossing and resistance gene transfer. 8. Remote crossing and resistance gene transfers 9. Biotechnological approaches in resistant breeding. Mutations and selection in vitro. 10. Use of recombinant DNA techniques in resistant breeding. 11. GMO plants - resistant to herbicides, insect pests. 12. Perspective transgenes in resistant breeding. 13. Induced systemic resistance - definition, biochemical nature of ISR. 14. Methods of ISR: Induction, detection, evaluation and persistence of induced resistance status.

Learning activities and teaching methods
Monologic (reading, lecture, briefing)
  • Semestral paper - 60 hours per semester
  • Preparation for credit - 20 hours per semester
  • Preparation for exam - 30 hours per semester
  • Class attendance - 56 hours per semester
Learning outcomes
The aim of the course is to clarify various aspects of the phenomenon of resistance, induced systemic resistance, and the possibility of plant breeding for resistance to biotic and abiotic stresses.
Students are able to orientate in the field of resistance plant breeding
Prerequisites
Standard knowledge of biology and breeding

Assessment methods and criteria
Combined exam

Active participation in exercises, protocols from laboratory exercises, understanding the curriculum defined for the exam.
Recommended literature
  • Al-Khayri, J.M. Advances in Plant Breeding Strategies: Breeding, Biotechnology and Molecular Tools, London, Springer, 656s, 2015..
  • David, P Clark; Nanette Jean Pazdernik. Biotechnology: applying the genetic revolution. Academic Press/Elsevier, 2009.
  • Novák, F. J. Explantátové kultury a jejich využití ve šlechtění rostlin. Academia, Praha, 1990..
  • Primrose, S.B. Molecular Biotechnology, 2. vydání Oxford, Blackwell Sci. Publ., 2000.
  • Seman, I. Biotechnologické metódy v šlachtení polných plodín. Príroda, Bratislava, 1990.
  • studijní materiály. http://biocentrum.zf.jcu.cz/ruzne.php.
  • Trigiano, R.N., Gray, D.J. Plant development and biotechnology. CRC Press Boca Raton, 358s. 2007..


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester