Course: Bacterial genetics

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Course title Bacterial genetics
Course code KMB/225
Organizational form of instruction Lecture
Level of course Doctoral
Year of study not specified
Frequency of the course In each academic year, in the winter semester.
Semester Winter
Number of ECTS credits 3
Language of instruction Czech
Status of course Compulsory-optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Petříčková Kateřina, Mgr. Ph.D.
  • Zrzavá Magda, RNDr. Ph.D.
Course content
The content of lectures: Structure of genomic DNA of prokaryotes, replication, transcription and translation specificities Mutation and suppression - principles, types, techniques Horizontal DNA transfers - conjugation, transduction and transformation, basic terminology, model mechanisms, methodology Genetics of basic cellular processes - cell division, morphological, physiological and metabolic differentiation of prokaryotes, interaction with a host in pathogenesis and symbiosis, inter-cellular signalization (biofilm, quorum sensing) Modern post-omic techniques in bacterial genetics - basic terminology, databases, software, application examples (details attached) Content of practicals: Competence, transformation, plasmids Mutant supplementation with precursors - model analysis of a biosynthetic pathway Interspecies conjugation - conjugative plasmids Transfection with phage DNA, isolation of phage DNA

Learning activities and teaching methods
Monologic (reading, lecture, briefing)
  • Preparation for classes - 30 hours per semester
  • Preparation for exam - 30 hours per semester
  • Class attendance - 26 hours per semester
Learning outcomes
Basics of bacterial genetics with the emphasis on its applications in common molecular biology techniques, bioinformatics and ecology
The student will get knowledge of basic bacterial genetics, genetic and molecular basis of control of cellular processes, and overview of modern techniques of bacterial genetics including examples of bioinformatic processing of -omic data.
Prerequisites
Basic understanding of microbiology and genetics.

Assessment methods and criteria
Written examination

To pass the exam, the student must score at least 20 points out of 30 from the final examination text.
Recommended literature
  • Dle vývoje v oboru doplněno o recentní články z odborných časopisů (max 5).
  • Snyder L., Peters J.E., Henkin T.M., Champness W.: Molecular genetics of bacteria 4th edition, ISBN-13: 978-1555816278 - vybrané kapitoly.
  • Yi-Wei Tang Y-W.,Sussman M., Liu D., Poxton I., Schwartzman J.: Molecular medical microbiology, ISBN: 978-0-12-397169-2 - pro genetiku lidských patogenů, jen vybrané kapitoly.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Science Study plan (Version): Experimental Biology (1) Category: Biology courses - Recommended year of study:-, Recommended semester: Winter