Course: Molecular Biology

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Course title Molecular Biology
Course code KMB/250
Organizational form of instruction Lecture
Level of course Bachelor
Year of study not specified
Frequency of the course In each academic year, in the winter semester.
Semester Winter
Number of ECTS credits 5
Language of instruction Czech
Status of course Compulsory, Compulsory-optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Bruce Alena, RNDr. Ph.D.
  • Zíková Alena, RNDr. Ph.D.
Course content
Content of lectures 1. Structure of the nucleic acids and proteins, structure of the genome, central dogma of molecular biology. 2. Replication of DNA in prokaryotes and eukaryotes. 3. Transcription in prokaryotes and eukaryotes 4. Regulation of transcription in prokaryotes 5. Regulation of transcription in eukaryotes 6. Splicing, transport, stability and editing of RNA, non-coding RNA 7. Proteins synthesis, genetic code, prions. 8. Structure of proteins, posttranslational modification. 9. Viruses, transposones. 10. Mutations, repair of DNA. 11. Basic methods of molecular biology. 12. Genomics.

Learning activities and teaching methods
Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Projection
  • Preparation for classes - 60 hours per semester
  • Class attendance - 36 hours per semester
  • Preparation for credit - 40 hours per semester
  • Preparation for exam - 40 hours per semester
Learning outcomes
To make students familiar with the principles of the genetic code and with the basic mechanisms of storing, expression and modification of genetic information
Student will understand the basic principles of molecular biology, in particular the molecular processes behind DNA replication, transcription, protein translation and reparation of DNA. Emphasis will be given to the understanding of regulatory mechanisms behind these processes both in prokaryotes and eukaryotes. Part of each lecture will be devoted to the principles of basic methods of molecular biology, like PCR, sequencing, gel electrophoresis, restriction analysis, RNA interference, Crispr/Cas9 and others.
Prerequisites
Participants of the course are expected to have a secondary school knowledge of chemistry and cell biology.

Assessment methods and criteria
Essay, Test

As a requirement for 'zapocet' the participants of the course are asked to take part of a miniconference regarding Nobel prize winners from the field of molecular biology. Student will make their own presentations to explain the key experiments that lead to the discoveries. It is strongly recommended to attend the lectures although it is not a compulsory requirement for the admission to the final test.
Recommended literature
  • Skripta: J. Vlasák: "Molekulární biologie I", http://kgn.umbr.cas.cz/kgn_250.html.
  • Alberts. Molecular biology of the cell. Taylor & Francis Books Ltd, 2014. ISBN 9780815344643.
  • Alberts. Základy buněčné biologie. Espero Publishing, 2005. ISBN 80-902906-2-0.
  • Clark, Pazdernik. Molecular biology, 2nd edition. Academic Cell, 2012. ISBN 9780123785954.
  • Watson. Molecular biology of the gene, 7th edition. Pearson Education (US), 2014. ISBN 0321762436.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Science Study plan (Version): Biology for future teachers (1) Category: Biology courses - Recommended year of study:-, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Biology for future teachers (1) Category: Biology courses - Recommended year of study:-, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Secondary Schools Teacher Training in Biology (1) Category: Pedagogy, teacher training and social care - Recommended year of study:-, Recommended semester: Winter
Faculty: Faculty of Education Study plan (Version): Biology Education (1) Category: Pedagogy, teacher training and social care - Recommended year of study:-, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Biology (1) Category: Biology courses - Recommended year of study:-, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Secondary Schools Teacher Training in Biology (1) Category: Pedagogy, teacher training and social care - Recommended year of study:-, Recommended semester: Winter
Faculty: Faculty of Education Study plan (Version): Biology Education (1) Category: Pedagogy, teacher training and social care - Recommended year of study:-, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Biomedical Laboratory Techniques (1) Category: Biology courses - Recommended year of study:-, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Experimental Biology (1) Category: Biology courses - Recommended year of study:-, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Biophysics (1) Category: Physics courses - Recommended year of study:-, Recommended semester: Winter