Lecturer(s)
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Petrzik Karel, prof. RNDr. CSc.
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Course content
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Content of lectures: Taxonomy, virus proteins, its function and evolution~Coat protein, primary and secondary structures, composition of virus particles~Replication and replicas~Strategies of expression of virus genes~Organization and expression of (+)RNA tobamoviruses, (-)ssRNA rhabdoviruses, ąRNA bunyaviruses, dsRNA reoviruses~Organization and expression of dsDNA caulimoviruses, ssDNA geminiviruses~Movement of viruses, role of movement proteins~Viral proteases, biotechnology with proteases~Interactions of viral genes with vectors~Subviral pathogens-viroids, virusoids, satRNA, ribozymes, DI RNA~Molecular interactions of viruses with hosts and vectors, plant resistance genes, pathogen-derived resistance~Preparation of resistant plants, resistance-breaking strains~Expression of heterologous peptides in coat protein of tobacco mosaic virus~Viruses as gene vectors - cowpea mosaic virus, potato virus X models Content of practices: Work with source databases, practical isolation of viral nucleic acids, RT-PCR and PCR detection of viruses, primer construction, mutagenesis of viruses, computer modelling of virus protein features.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Projection, Laboratory, Experiment
- Preparation for classes
- 45 hours per semester
- Preparation for exam
- 50 hours per semester
- Class attendance
- 30 hours per semester
- Preparation for credit
- 25 hours per semester
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Learning outcomes
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To acquaint students with plant virus genome functions, show methods of transgenic protection against plant viruses and the biotechnological use of plant viruses.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Combined exam
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Recommended literature
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J. Hammond, P. McGarvey, V. Yusibov - Plant Biotechnology, Springer Verlag,Berlin, 1999.
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K. Petrzik - Molekulární biologie a genetika rostlinných virů, ÚMBR, skripta, 2000.
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R. Hull - Matthews´ Plant Virology, Academic Press, 2002.
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S. Rosypal - Úvod do molekulární biologie, díl 4., kap. 17, S. Rosypal nákladem vlastním, Brno 2002.
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