Course: Molecular Genetics of Plant Viruses

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Course title Molecular Genetics of Plant Viruses
Course code KMB/256
Organizational form of instruction Lecture + Lesson
Level of course Bachelor
Year of study 2
Frequency of the course In academic years starting with an even year (e.g. 2016/2017), in the summer semester.
Semester Summer
Number of ECTS credits 5
Language of instruction Czech
Status of course Compulsory-optional
Form of instruction unspecified
Work placements unspecified
Recommended optional programme components None
Lecturer(s)
  • Petrzik Karel, prof. RNDr. CSc.
Course content
Content of lectures: Taxonomy, virus proteins, its function and evolution~Coat protein, primary and secondary structures, composition of virus particles~Replication and replicas~Strategies of expression of virus genes~Organization and expression of (+)RNA tobamoviruses, (-)ssRNA rhabdoviruses, ąRNA bunyaviruses, dsRNA reoviruses~Organization and expression of dsDNA caulimoviruses, ssDNA geminiviruses~Movement of viruses, role of movement proteins~Viral proteases, biotechnology with proteases~Interactions of viral genes with vectors~Subviral pathogens-viroids, virusoids, satRNA, ribozymes, DI RNA~Molecular interactions of viruses with hosts and vectors, plant resistance genes, pathogen-derived resistance~Preparation of resistant plants, resistance-breaking strains~Expression of heterologous peptides in coat protein of tobacco mosaic virus~Viruses as gene vectors - cowpea mosaic virus, potato virus X models Content of practices: Work with source databases, practical isolation of viral nucleic acids, RT-PCR and PCR detection of viruses, primer construction, mutagenesis of viruses, computer modelling of virus protein features.

Learning activities and teaching methods
Monologic (reading, lecture, briefing), Projection, Laboratory, Experiment
  • Preparation for classes - 45 hours per semester
  • Preparation for exam - 50 hours per semester
  • Class attendance - 30 hours per semester
  • Preparation for credit - 25 hours per semester
Learning outcomes
To acquaint students with plant virus genome functions, show methods of transgenic protection against plant viruses and the biotechnological use of plant viruses.

Prerequisites
unspecified

Assessment methods and criteria
Combined exam

Recommended literature
  • J. Hammond, P. McGarvey, V. Yusibov - Plant Biotechnology, Springer Verlag,Berlin, 1999.
  • K. Petrzik - Molekulární biologie a genetika rostlinných virů, ÚMBR, skripta, 2000.
  • R. Hull - Matthews´ Plant Virology, Academic Press, 2002.
  • S. Rosypal - Úvod do molekulární biologie, díl 4., kap. 17, S. Rosypal nákladem vlastním, Brno 2002.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester