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Lecturer(s)
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Tolar Nikolas, Mgr.
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Husová Michaela, Mgr.
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Moos Martin, Ing. Ph.D.
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Bittová Lenka, Mgr. Ph.D.
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Šauman Ivo, prof. RNDr. Ph.D.
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Korytář Tomáš, Mgr. Ph.D.
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Dalíková Martina, RNDr. Ph.D.
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Gahurová Lenka, Mgr. Ph.D.
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Doležal Tomáš, doc. Mgr. Ph.D.
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Mozgová Iva, Mgr. Ph.D.
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Panicucci Zíková Alena, doc. RNDr. Ph.D.
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Nguyen Petr, RNDr. Ph.D.
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Hospodářská Monika, Mgr.
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Chodáková Lenka, Mgr. Ph.D.
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Bajgar Adam, Mgr. Ph.D.
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Sehadová Hana, Mgr. Ph.D.
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Krejčová Gabriela, RNDr. Ph.D.
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Jindra Marek, prof. RNDr. CSc.
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Fencková Michaela, Mgr. Ph.D.
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Podlahová Šárka, Mgr.
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Hrubá Monika, Mgr.
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Tůmová Šárka
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Course content
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Content of lectures: 1. Advanced genetic tools in Drosophila model 2. Protein expression analysis, Western blotting 3. Protein tagging and proteomics 4. Immunohistochemistry and antibody production 5. Cell transfection, cell culture 6. Targeted mutagenesis, CRISPR/Cas9 7. Next generation sequencing methods - genome assembly and comparisons 8.Next generation sequencing methods in gene expression and epigenetics 9. 13C biology and metabolomics 10. Flow cytometry and cell sorting
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Monitoring, Demonstration, Laboratory, Experiment
- Preparation for classes
- 75 hours per semester
- Class attendance
- 95 hours per semester
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Learning outcomes
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This Specialized course is for Master and PhD students working in the field of molecular biology (or related areas of experimental biology). The goal is to present advanced methods used in molecular and cell biology research.
Absolvents of this course should be able to understand most of the molecular-biology techniques not only in the scientific field. Students should be able to design experiments independently and serve as an advisors for student from other than molecular-biology fields.
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Prerequisites
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Student should have a good knowledge of basic molecular biology techniques. Further they should be able to orient in the most of the moleculab and developmental biology terms and in basic genetics. Ability to think independently, motivation to learn something new on your own, fundamental knowledge of the methods discussed in the course Basic Methods of Molecular Biology KMB 608.
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Assessment methods and criteria
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Written examination, Development of laboratory protocols
To successfully complete the course, it is necessary to pass the written test with min. 50% and it is necessary to participate in 2 selected practices and prepare protocols from them according to the instructions of the specific supervisor.
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Recommended literature
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Sambrook, J., Fritsch, E. J. and Maniatis, T. (1989) Molecular Cloning, A Laboratory Manual (2. vyd. ), Cold Spring Harbor Press..
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Ochrana František. Methodology of science : an introduction.
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ONLINE zdroje. ONLINE MATERIÁLY.
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