| Course title | Advanced Methods in Molecular Biology |
|---|---|
| Course code | KMB/603 |
| Organizational form of instruction | Lecture + Lesson |
| Level of course | Master |
| Year of study | not specified |
| Frequency of the course | In each academic year, in the summer semester. |
| Semester | Summer |
| Number of ECTS credits | 6 |
| Language of instruction | Czech |
| Status of course | Compulsory, Compulsory-optional |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
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| Course content |
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Content of lectures: 1. Advanced genetic tools in Drosophila model 2. Protein expression analysis, Western blotting 3. Protein tagging and proteomics 4. Immunohistochemistry and antibody production 5. Cell transfection, cell culture 6. Targeted mutagenesis, CRISPR/Cas9 7. Next generation sequencing methods - genome assembly and comparisons 8.Next generation sequencing methods in gene expression and epigenetics 9. 13C biology and metabolomics 10. Flow cytometry and cell sorting
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| Learning activities and teaching methods |
Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Monitoring, Demonstration, Laboratory, Experiment
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| Learning outcomes |
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This Specialized course is for Master and PhD students working in the field of molecular biology (or related areas of experimental biology). The goal is to present advanced methods used in molecular and cell biology research.
Absolvents of this course should be able to understand most of the molecular-biology techniques not only in the scientific field. Students should be able to design experiments independently and serve as an advisors for student from other than molecular-biology fields. |
| Prerequisites |
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Student should have a good knowledge of basic molecular biology techniques. Further they should be able to orient in the most of the moleculab and developmental biology terms and in basic genetics.
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| Assessment methods and criteria |
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Written examination
To pass the practical part of the course, students must attend two selected practical sessions. |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Science | Study plan (Version): Molecular and Cell Biology and Genetics (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Fyziologie a vývojová biologie (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Infection Biology (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Infection Biology (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Molecular and Cell Biology and Genetics (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Parasitology (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Parasitology (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Physiology and Developmental Biology (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Experimental Biology (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |