Course: Basic Methods of Molecular Biology

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Course title Basic Methods of Molecular Biology
Course code KMB/608
Organizational form of instruction Lecture + Lesson
Level of course Bachelor
Year of study not specified
Frequency of the course In each academic year, in the winter semester.
Semester Winter
Number of ECTS credits 8
Language of instruction Czech
Status of course Compulsory, Compulsory-optional, Optional
Form of instruction unspecified
Work placements unspecified
Recommended optional programme components None
Lecturer(s)
  • Kunzová Michaela, Mgr.
  • Krejčová Gabriela, Mgr.
  • Bajgar Adam, Mgr. Ph.D.
Course content
Content of lectures: INTRODUCTION 1. Basic orientation in molecular lab - equipment, chemicals and preparation of reagents. 2. Working with sequences - basic rules and terms, databases and working with sequences in computer. BLOCK I - basic methodology including technical details 3. Isolation of various types of nucleic acids; gel electrophoresis 4. DNA cloning - various approaches, vectors, restriction endonucleases, cloning design 5. PCR and its applications 6. Genetically modified organisms - safety training. 7. Nucleic acids labelling and detection, hybridization, blotting 8. DNA sequencing, "next generation sequencing" BLOCK II - advanced approaches - demonstration of principles 9. Polymorphism and genetic diversity analysis, homologous genes identification 10. Principles of expression analysis at RNA and protein levels 11. Proteomics, metabolomics, mass spectroscopy Content of practicals: Isolation of genomic DNA and total RNA Reverse transcription to cDNA PCR amplification from both genomic DNA and cDNA, gel electrophoresis TA-cloning and bacterial transformation Blue-white selection Plasmid DNA isolation Restriction analysis of purified plasmid Cloned fragment sequencing Determination of sequence by BLAST

Learning activities and teaching methods
Demonstration, Projection, Laboratory
  • Preparation for credit - 35 hours per semester
  • Preparation for classes - 60 hours per semester
  • Class attendance - 120 hours per semester
Learning outcomes
Objective of the course is to introduce basic molecular biology techniques to students without regarding their orientation either to experimental or so called "green" biology. The molecular techniques which are commonly used by all biology fields will be presented. The course is suitable for students who passed Basic Cell Biology, Genetics and Biochemistry. It is recommended also to students who are already working in molecular biology labs but their knowledge is limited to the small number of techniques used in that particular lab.
Absolvents of this course will gain abilities to understand most of the molecular techniques used commonly in molecular-biological laboratories. We pay an attention on improvement of methodological thinking and competencies important for designing of experiments and their practical realization. Students should be able to orient themselves in wide variety of molecular- biological techniques and understand principles and troubleshooting. Important part of the course is practical experience in the lab, where students get familiar with basic laboratory analysis of DNA and RNA molecules.
Prerequisites
Basic understanding of principles of cellular biology. Understanding of transcription and translation as well as structure of basic macromolecules - DNA, RNA, proteins. Understanding of genetic code and composition of DNA information.

Assessment methods and criteria
Oral examination, Written examination

Students have to gain 75% in the test at the end of the practical course to can come for oral examination. During oral examination, students have to convince the examiner about their ability to orient in the methods associated with their project and to critically and methodically think about projects of other students and scientific groups.
Recommended literature
  • ONLINE ZDROJE.
  • ŠMARDA, DOŠKAŘ, PANTUČEK, RUŽIČKOVÁ, KOPTÍKOVÁ, Jana. Metody molekulární biologie. Vyd. 1. Brno: Masarykova univerzita, 2005.
  • Alberts a kol.. Základy buněčné biologie. 2008.
  • Ochrana František. Methodology of science : an introduction. 2012.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Education Study plan (Version): Biology Education (1) Category: Pedagogy, teacher training and social care - Recommended year of study:-, Recommended semester: Winter
Faculty: Faculty of Agriculture Study plan (Version): Agricultural Biotechnology (2013) Category: Agriculture and forestry - Recommended year of study:-, Recommended semester: Winter
Faculty: Faculty of Agriculture Study plan (Version): Agricultural Biotechnology (2014) Category: Agriculture and forestry - Recommended year of study:-, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Secondary Schools Teacher Training in Biology (1) Category: Pedagogy, teacher training and social care - Recommended year of study:-, Recommended semester: Winter
Faculty: Faculty of Agriculture Study plan (Version): Agricultural Biotechnology (2014) Category: Agriculture and forestry - Recommended year of study:-, Recommended semester: Winter
Faculty: Faculty of Education Study plan (Version): Biology Education (1) Category: Pedagogy, teacher training and social care - Recommended year of study:-, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Secondary Schools Teacher Training in Biology (1) Category: Pedagogy, teacher training and social care - Recommended year of study:-, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Experimental Biology (1) Category: Biology courses - Recommended year of study:-, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Clinical Biology (1) Category: Biology courses - Recommended year of study:-, Recommended semester: Winter