Course: Molecular Genetics of Model Organisms and Human

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Course title Molecular Genetics of Model Organisms and Human
Course code KMB/696
Organizational form of instruction Lecture + Lesson
Level of course Master
Year of study not specified
Frequency of the course In each academic year, in the winter semester.
Semester Winter
Number of ECTS credits 6
Language of instruction Czech
Status of course Compulsory-optional
Form of instruction unspecified
Work placements unspecified
Recommended optional programme components None
Lecturer(s)
  • Žurovec Michal, prof. RNDr. CSc.
Course content
Content of lectures 1) The paradigm of model organisms (forward and reverse genetics, mutagenesis, genome sequencing, transgenes, inbred lines, epistasis analysis, allelic variation, gene testing). 2) Introduction to human genetics (pedigree analysis of inheritance of dominant and recessive genes, linkage analysis, linkage disequilibrium and LOD score, human sequencing project). 3) Mendelian diseases (instructive examples of specific disorders to illustrate genome wide scans, haplotypes, homozygosity mapping, strategies for positional cloning, prenatal diagnostics). 4) Current human genetic research topics (complex disease loci, HapMap project, SNP mapping, stem cells, gene therapy). 5) Yeast and its contribution to the understanding of eukaryotic biology. Molecular analysis of the cell cycle. 6) Cancer genetics (evolution of cancer, oncogenes, tumor suppressors and DNA repair genes, mutation/rearrangement detection systems, loss of heterozygosity, epigenetic silencing, translocations, genomic instability and cancer, cancer stem cells). 7) Murine genetics (transgenics, knockouts, conditionals, ENU and insertional mutagenesis screens, identifying modifier genes, haplotype-based genetic analysis, mouse strains, congenics, recombinant inbreds, advanced intercrosses, quantitative trait loci mapping). 8) Genetics of Drosophila (chromosomal rearrangements, saturation mutagenesis, the logic of genetic screenings, genetic dissection of early development, developmental genetics, behavioural mutants, elucidation of gene regulatory networks). 9) Optinal lecture (choice from 3 topics: "C. elegans", "microarrays" or "GFP as a universal marker"). Content of practicals a) Preparation of presentation with topic coming out from one of the lectures and with using scientific paper in outstanding impacted journal. b) Poster preparation with topic coming out from one of the lectures and with using scientific paper in outstanding impacted journal.

Learning activities and teaching methods
Dialogic (discussion, interview, brainstorming)
  • Preparation for classes - 10 hours per semester
  • Class attendance - 36 hours per semester
  • Preparation for exam - 45 hours per semester
Learning outcomes
The main objective of the course is to introduce the broad range of research topics in current molecular genetic studies. We will discuss limitations and disadvantages of using human in genetic analysis, explain roles of experimentally tractable model organisms to study human gene function. We will introduce major genetic model organisms, emphasizing both the similarities and the particular strengths of each model.
The course provides important background information for conducting advanced basic research.
Prerequisites
To enroll in this course, students should have completed introductory cell biology, and molecular biology prerequisite courses. Special course for Ph.D. study field Molecular and Cell Biology and Genetics

Assessment methods and criteria
Student performance assessment

The exam is in the form of multiple-choice test and comprises 20 questions.
Recommended literature
  • An Introduction to Genetic Analysis by Anthony J. F. Griffiths, Jeffrey H. Miller, David T.Suzuki, Richard C. Lewontin, and William M. Gelbart. W.H. Freeman and Comp..
  • Molekulární genetika modelových organismů - skripta v elektronické formě: (obnovená verze skript Molekulární biologie živočichů).
  • Philip Meneely. Advanced Genetic Analysis. 2009. ISBN 978-0-19-921982-7.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Science Study plan (Version): Experimental Biology (1) Category: Biology courses - Recommended year of study:-, Recommended semester: Winter