Course: Molecular Biology and Genetics I

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Course title Molecular Biology and Genetics I
Course code KMB/758
Organizational form of instruction Lecture
Level of course Bachelor
Year of study not specified
Frequency of the course In each academic year, in the winter semester.
Semester Winter
Number of ECTS credits 3
Language of instruction English
Status of course Compulsory-optional
Form of instruction unspecified
Work placements unspecified
Recommended optional programme components None
Course availability The course is available to visiting students
  • Gahurová Lenka, Mgr. Ph.D.
  • Bruce Alexander William, doc. Ph.D.
  • Stiborová Martina, Mgr.
  • Collier Rebecca, MSc.
Course content
Content of lectures: Introduction to molecular biology: History and application of molecular biology in today's science. Molecular structure of genes and chromosome: Molecular definition of a gene, chromosomal organization of genes and noncoding DNA, structural organization of chromosomes. Basic molecular genetic mechanisms, Replication of DNA: Understanding the central dogma of molecular biology and overview of basic molecular genetic mechanisms. Basic features of DNA replication in vivo. Transcription and RNA processing, Translation and the genetic code: Principles of transcription in Prokaryotes and Eukaryotes, basic regulation of gene expression at transcriptional and postranscriptional level. Principles of translation, genetic code. Mutation, DNA repair, and recombination: Molecular basis of mutations, induced mutagenesis, overview of repair mechanisms and principles of recombination. Regulation of gene expression in Prokaryotes, Eukaryotes and the genetic control of development: Operon, molecular control of transcription in Eukaryotes, gene expression and chromosome organization, mechanisms of regulation of gene expression during development. Molecular analysis of genes and gene products: Use of recombinant DNA technology to identify genes, molecular diagnosis of human diseases. Recombinant DNA technology, the polymerase chain reaction: Cloning genes, principle of polymerase chain reaction and its applications. Basic techniques of molecular biology: Overview of molecular biology techniques and their use in modern research. Overview of molecular biology applications in modern basic and applied science: Summary of the course in context of using molecular biology in modern research.

Learning activities and teaching methods
Monologic (reading, lecture, briefing), Work with text (with textbook, with book)
  • Preparation for classes - 20 hours per semester
Learning outcomes
The subject aims to be an introduction into molecular biology of the cell. Basic molecular biology mechanisms in cells will be reviewed and overview of applications of molecular biology techniques in today's science will be given.


Assessment methods and criteria
Written examination, Test

Recommended literature
  • Bruce Alberts et al.,: Molecular Biology of the Cell - Fifth Edition onwards, Taylor & Francis Books Ltd..
  • D. Peter Snustad and Michael J. Simmons: Principles of Genetics, Wiley.
  • David P. Clark: Molecular Biology Understanding the Genetic Revolution, Academic Cell..
  • Harvey Lodish et al.: Molecular Cell Biology - Fifth Edition, W.H. Freeman, 2004.

Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Science Study plan (Version): Biological Chemistry (1) Category: Chemistry courses 2 Recommended year of study:2, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Bioinformatics (1) Category: Informatics courses 1 Recommended year of study:1, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Experimental Biology (1) Category: Biology courses - Recommended year of study:-, Recommended semester: Winter