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Lecturer(s)
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Chodáková Lenka, Mgr. Ph.D.
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Miláček Matěj, Mgr.
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Wong Johnatan
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Dolejšková Tereza, Mgr.
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Course content
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Content of the course 1. Orientation in the molecular biology laboratory 2. Isolation of genomic DNA (gDNA), isolation of RNA 3. Gel electrophoresis 4. Reverse transcription 5. Polymerase chain reaction (PCR) 6. Cloning - ligation of the PCR product with the plasmid 7. Screening of recombinants 8. Isolation of plasmid DNA (pDNA) 9. Sequencing (theoretical) 10. Analysis of the results, guides for "How to make a protocol".
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Monitoring, Demonstration, Projection, Laboratory, Experiment, Group work
- Class attendance
- 45 hours per semester
- Preparation for exam
- 30 hours per semester
- Preparation for classes
- 25 hours per semester
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Learning outcomes
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This course is primarily intended for bachelor students of joint Linz-Ceske Budejovice - Biological chemistry - program. The students will get acquainted with basic laboratory work and molecular biology methods.
Students will be able to perform basic methods in molecular biology in laboratories, as nucleic acid isolation, PCR, cloning and gel electrophoresis.
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Prerequisites
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It is recommended to attend the KMB 219 course.
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Assessment methods and criteria
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Student performance assessment, Systematic student observation, Test, Development of laboratory protocols
Active participation in lessons, absences possible only by prior arrangement. At the end of the course the student will prepare a report on the laboratory work.
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Recommended literature
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Alberts et al.: Molecular Biology of the Cell..
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Sambrook, J., Fritsch, E. J. and Maniatis, T. (1989) Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Press..
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