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Lecturer(s)
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Litvín Radek, doc. RNDr. Ph.D.
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Course content
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1. Structure of Matter - Atoms, Molecules, Biological Polymers 2. Types of Radiation and Their Interaction with Matter 3. Forms of Energy and Their Transformations, Basics of Thermodynamics 4. Physical Properties of Water and Their Biological Significance 5. Non-Covalent Interactions in Biology 6. Methods for Measuring the Kinetics of Biological Processes 7. Structure and Function of Biological Membranes 8. Electrochemical Processes in Biological Systems 9. Bioenergetics 10. Membrane-Associated Biological Functions: Biochemistry of Vision, Respiratory Chain, and Photosynthesis 11. Use of Optical Analytical Methods for Substance Detection and Quantification 12. Fluorescence-Based Detection and Analytical Methods, Fluorimetry 13. Methods for Studying the Spatial Structure of Biopolymers - NMR and X-ray Crystallography, Circular Dichroism 14. Use of Radioisotopes in the Study of Biological Processes
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Work with multi-media resources (texts, internet, IT technologies)
- Class attendance
- 26 hours per semester
- Preparation for classes
- 26 hours per semester
- Preparation for exam
- 26 hours per semester
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Learning outcomes
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The aim of the course is to provide students with the physical and physico-chemical knowledge necessary for the application of physical methods in clinical laboratory diagnostics. The course introduces key physical principles that underpin modern diagnostic techniques used in laboratory medicine. It focuses on the application of electromagnetic radiation, optics, spectroscopy, nuclear and quantum physics in the medical environment. Emphasis is placed on understanding the function and interpretation of results obtained from instrument-based methods that utilize modern technologies. *** The course is primarily intended for students of the Bachelor's program in Laboratory and Medical Biology and will be offered as an elective for those interested in the follow-up professional Master's program in Bioanalytical Laboratory Diagnostics in Healthcare, where biophysics in this scope and content is required by the regulator - the Ministry of Health.
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Prerequisites
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The student should have completed the courses Obecná a fyzikální chemie a Fyzika pro biology or equivalent knowledge and competence.
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Assessment methods and criteria
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unspecified
Student must give at least 50% of correct answers in oral exam or written test
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Recommended literature
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ATKINS, P. W. a DE PAULA, Julio. Fyzikální chemie. Praha: Vysoká škola chemicko-technologická v Praze, 2013. ISBN 978-80-7080-830-6..
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KODÍČEK, Milan a KARPENKO, Vladimír. Biofysikální chemie. Vydání 3., opravené a rozšířené. Praha: Academia, 2013. ISBN 978-80-200-2241-7.
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PROSSER, Václav. Experimentální metody biofyziky. Praha: Academia, 1989. ISBN 80-200-0059-3.
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ROSINA, Jozef; VRÁNOVÁ, Jana a KOLÁŘOVÁ, Hana. Biofyzika: pro zdravotnické a biomedicínské obory. 2., doplněné vydání. Praha: Grada Publishing, 2021. ISBN 978-80-271-2526-5.
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