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Lecturer(s)
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Langhansová Helena, RNDr. Ph.D.
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Course content
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Content of lectures: 1. History, terms and definitions, system of animal protection in the Czech Republic, ethical and economic aspects, 3R principle, . 2. Biological experimental models - experimental vs. laboratory animals, alternative models (invertebrates, microorganisms, cell and tissue cultures). Factors affecting animal quality.Consumption of experimental animals in the Czech Republic. Particularities of the experiments on farm and free-living animals. 3. Genetics of the laboratory animals (inbreeding, congenic strains, genetically modified organisms). SPF and barrier breeding. Gnotobiology. 4. Biology and physiology of laboratory animals. 5. Diseases of laboratory animals and diagnostics. Animal hygiene. 6. Experimental procedures (manipulation, collection of biological material, drug application, anesthesia, euthanasia, perfusion fixation). 7. Animal models of human disorders (atherosclerosis, obesity, asthma, multiple sclerosis etc.). Humanized mice. 8. Legislation (Act on animal protection against cruelty, Regulation over protection of experimental animals). 9. Experimental project. 10. Excursion to animal facilities of Faculty of Science, University of South Bohemia. 11.-12. Reviews and discussion.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Excursion
- Class attendance
- 26 hours per semester
- Preparation for classes
- 21 hours per semester
- Preparation for exam
- 33 hours per semester
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Learning outcomes
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The main objective of the course is theoretical and organizational training of students for the prospective experiments on animals within their master and Ph.D. theses. The students obtain general knowledge about various experimental models, as well as about related legislative regulations and proper experimental project.
The graduates have general knowledge about basic principles, pros and cons of animal experiments. They know related legislation and ethic issues. They are able to prepare the experimental project properly.
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Prerequisites
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The course requires basic knowledge about the anatomy and physiology of vertebrates. It is highly reccomended to attend Immunology (KME/216) in advance or concurrenly with this course.
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Assessment methods and criteria
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Written examination, Student performance assessment
Short presentation (approx. 10 min.) of a selected topic Written examination test (50 % pass) This course does not replace the Specialized training for acquiring expertise and special qualification according to § 15d Act no. 246/1992 Coll. on animal protection against cruelty.
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Recommended literature
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GLUCK, John P., Tony DIPASQUALE a F. Barbara. ORLANS. Applied ethics in animal research: philosophy, regulation, and laboratory applications. West Lafayette, Ind.: Purdue University Press, c2002. ISBN 1557531374..
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JEBAVÝ, Lukáš. Chov laboratorních zvířat. Praha, Česká zemědělská univerzita, 2011. ISBN 978-80-213-2178-6..
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Vyhláška o ochraně pokusných zvířat (419/2012 Sb., ve znění pozdějších předpisů).
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Zákon na ochranu zvířat proti týrání (246/1992 Sb., ve znění pozdějších předpisů).
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ZUTPHEN, L.F.M. van, BAUMANS V. and BEYNEN A.C. (Eds.). Principles of laboratory animal science: a contribution to the humane use and care of animals and to the quality of experimental results. Rev. ed. New York: Elsevier, 2001. ISBN 0444506128..
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JEBAVÝ, L., RODL, P., HOŘAVOVÁ, P. (Ed.). Ochrana, chov a využití pokusných zvířat. Brno, SVLZ, 2005.
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