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Lecturer(s)
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Kamiš Jan, RNDr.
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Doudová Lenka, Mgr.
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Palus Martin, RNDr. Ph.D.
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Hönig Václav, Mgr. Ph.D.
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Kotounová Eliška, Mgr. MSc.
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Prokopová Daniela, MUDr.
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Kříha Michal, MUDr.
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Lieskovská Jaroslava, doc. Mgr. Ph.D.
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Prančlová Veronika, Mgr.
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Kopecký Jan, prof. RNDr. CSc.
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Kovaříková Anna, Mgr.
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Course content
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Content of lectures: Introductory to virology and to the study of virus-host cells interactions including historical survey of the main discoveries in virology. 2 The origin and the nature of viruses: Evolution of intracellular parasitism, developmental cycles of viruses. 3 Physical morphology of viruses: Thermodynamics and crystalography of viruses. 4. Chemical morphology of viruses: Structural and non-structural components of viruses. 5 Functional morphology of viruses: Functional features of structural and non-structural components. 6 Molecular principles of virus reproduction I: Viral genomes, their polarity and interactions with genomes of the host cells, replication mechanisms of viral genomes, translation of viral proteins and their posttranslational processing. 7 Molecular principles of virus reproduction II: Replication strategies of viral genomes. 8 Mechanisms of virus-host cell interaction I: Virus specific receptors/ signalling. 9 Mechanisms of virus-host cell interaction II: Molecular mechanisms of viral pathogenesis of infected cells, factors of virulence). 10 Interaction mechanisms in infectious systems of virus-tissue and virus-organism: Pathogenesis of viral infections of the hosts. 11 Methods in the research of virus-host cell interaction: Fundametal techniques on analytical/molecular and preparative virology. 12 Taxonomy of viruses I: Viruses of prokaryotic cells. 13 Taxonomy of viruses II: Viruses of eukaryotic cells 14 Introductory to molecular ecologv of viruses: Subcellular and molecular factors of inner environment of the host cell affecting intracellular parasitism of the virus. Seminar content (30 minutes just after lecture): 1. Introduction: introductory information, programme, topics 2. Student´s seminar - individual work with literature, presentation of results (Nobel Prize laureates etc.) 3. Case reporst of human virus infections from clinical practice 4. Fundamentals of manipulation with infectious material, classification of viosafety levels, classification of iological agents 5. Theory of basic virology methods (just before practicals) 6. Interactive seminar: video-illustation of epidemy/pandemy development - reality versus fiction (Contagion 2011, Outbreak 1995) Content of practical part of course: 1. Determination of virus titer by method of plaque titration. Calculation of viral titer. 2. Usage of in vivo models in virology. 3. Virus infection of cell culture + monitoring of cytopathic effect. 4. Immunofluorescent detection of viral proteins in cellular culture and virus neutralization test. 5. Excursion at Department of Virology in Hospital in České Budějovice.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Work with text (with textbook, with book), Projection, Individual preparation for exam
- Preparation for classes
- 24 hours per semester
- Preparation for exam
- 50 hours per semester
- Class attendance
- 36 hours per semester
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Learning outcomes
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The course provides a fundamental knowledge of biology and molecular biology of viruses with the respect to their interaction with the host cells. The main attention of the course is focused on snímal/human viruses including their developmental cycles, physical, chemical and functional morphology, molecular aspects of their reproduction, mechanisms of interactions with permissive cells, taxonomy of viruses and methods in virological research. Epidemiology and imunology of viral diseases of men and vertebrates.
Student has a basic information about viruses, their structure, molecular biology and genetics. Student understands the mechanims of replication of several types of animal viruses in the host cells. Student is well oriented in the taxonomy of human viruses and knows the causative agenst of the most important human viral diseases. Student undertands basic mechanims of viral pathogenesis. Student knows the possibilities of prevention and therapy of viral diseases.
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Prerequisites
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Student should have a basic knowledge in the field of molecular and cellular biology, and microbiology.
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Assessment methods and criteria
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Test
Student requirements: Presence at seminars 75 %, presence at laboratory practicals 75 % (obligatory for the credit). Exam pass - min. 60 % points in written test.
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Recommended literature
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B.W.J. Mahy: A Dictionary of Virology. Academic Press, Cambridge, 1997..
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H-W.Ackermann, L. Berthiaume, M. Tremblay: Virus Life in Diagrams. CRC Press, 1998..
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J. Žemla, F. Čiampor, L. Leššo: General virology. Slovak Academic Press, Bratislava, 1995, (in Slovak)..
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J. Žemla, F. Čiampor, M. Labuda: Systematic Virology. Slovak Academic Press, Bratislava, 1998, (in Slovak)..
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N.J. Dimmock, A.J. Easton, K.N. Leopard: Introduction to Modern Virology. 5th edition, Blackwell Science, 2001..
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R. Q. Webster, A. Granoff: Encyclopedia of virology, Vol. 1,2,3, Academic Press, 1994..
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