Course: General Virology

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Course title General Virology
Course code KME/603
Organizational form of instruction Lecture + Lesson
Level of course Master
Year of study not specified
Frequency of the course In each academic year, in the summer semester.
Semester Summer
Number of ECTS credits 6
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Grubhoffer Libor, prof. RNDr. CSc.
  • Růžek Daniel, prof. RNDr. Ph.D.
Course content
Content of lectures: Introductory to virology and to the study of virus-host cells interactions including historical survey of the main discoveries in virology. 2 The origin and the nature of viruses: Evolution of intracellular parasitism, developmental cycles of viruses. 3 Physical morphology of viruses: Thermodynamics and crystalography of viruses. 4. Chemical morphology of viruses: Structural and non-structural components of viruses. 5 Functional morphology of viruses: Functional features of structural and non-structural components. 6 Molecular principles of virus reproduction I: Viral genomes, their polarity and interactions with genomes of the host cells, replication mechanisms of viral genomes, translation of viral proteins and their posttranslational processing. 7 Molecular principles of virus reproduction II: Replication strategies of viral genomes. 8 Mechanisms of virus-host cell interaction I: Virus specific receptors/ signalling. 9 Mechanisms of virus-host cell interaction II: Molecular mechanisms of viral pathogenesis of infected cells, factors of virulence). 10 Interaction mechanisms in infectious systems of virus-tissue and virus-organism: Pathogenesis of viral infections of the hosts. 11 Methods in the research of virus-host cell interaction: Fundametal techniques on analytical/molecular and preparative virology. 12 Taxonomy of viruses I: Viruses of prokaryotic cells. 13 Taxonomy of viruses II: Viruses of eukaryotic cells 14 Introductory to molecular ecologv of viruses: Subcellular and molecular factors of inner environment of the host cell affecting intracellular parasitism of the virus. Content of practices: Cultivation methods in virology ; Tissue/Cell culture techniques ; Animal virus titration techniques; Serological techniques; Hemagglutination and inhibition hemagglutination techniques ; Molecular diagnostics-PCR methods ; Interference in viral infections, Ultrastructural studies-electron microscopy in virology; Excursion to the virological laboratory in the Hospital

Learning activities and teaching methods
Monologic (reading, lecture, briefing), Work with text (with textbook, with book), Projection, Individual preparation for exam
  • Preparation for classes - 24 hours per semester
  • Preparation for exam - 50 hours per semester
  • Class attendance - 36 hours per semester
Learning outcomes
The course provides a fundamental knowledge of biology and molecular biology of viruses with the respect to their interaction with the host cells. The main attention of the course is focused on snímal/human viruses including their developmental cycles, physical, chemical and functional morphology, molecular aspects of their reproduction, mechanisms of interactions with permissive cells, taxonomy of viruses and methods in virological research. Epidemiology and imunology of viral diseases of men and vertebrates.
Student has a basic information about viruses, their structure, molecular biology and genetics. Student understands the mechanims of replication of several types of animal viruses in the host cells. Student is well oriented in the taxonomy of human viruses and knows the causative agenst of the most important human viral diseases. Student undertands basic mechanims of viral pathogenesis. Student knows the possibilities of prevention and therapy of viral diseases.
Prerequisites
Student should have a basic knowledge in the field of molecular and cellular biology, and microbiology.

Assessment methods and criteria
Test

To pass an exam is needed to obtain at least 60% points in written exam.
Recommended literature
  • B.W.J. Mahy: A Dictionary of Virology. Academic Press, Cambridge, 1997..
  • H-W.Ackermann, L. Berthiaume, M. Tremblay: Virus Life in Diagrams. CRC Press, 1998..
  • J. Žemla, F. Čiampor, L. Leššo: General virology. Slovak Academic Press, Bratislava, 1995, (in Slovak)..
  • J. Žemla, F. Čiampor, M. Labuda: Systematic Virology. Slovak Academic Press, Bratislava, 1998, (in Slovak)..
  • N.J. Dimmock, A.J. Easton, K.N. Leopard: Introduction to Modern Virology. 5th edition, Blackwell Science, 2001..
  • R. Q. Webster, A. Granoff: Encyclopedia of virology, Vol. 1,2,3, Academic Press, 1994..


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Science Study plan (Version): Clinical Biology (1) Category: Biology courses - Recommended year of study:-, Recommended semester: Summer