Course: Animal Cell and Tissue Culture

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Course title Animal Cell and Tissue Culture
Course code KME/606
Organizational form of instruction Lesson
Level of course Bachelor
Year of study 2
Frequency of the course In each academic year, in the winter semester.
Semester Winter
Number of ECTS credits 3
Language of instruction Czech
Status of course Compulsory-optional
Form of instruction unspecified
Work placements unspecified
Recommended optional programme components None
Lecturer(s)
  • Kopecký Jan, prof. RNDr. CSc.
  • Výletová Eva, Ing.
Course content
1. Types of tissue cultures, cell growth parameters 2. Nutritional requirements, culture media 3. Tissue culture laboratory equipment 4. Primary culture of guinea pig kidney 5. Cell lines, passaging and culture conditions 6. Cell cryopreservation 7. Contamination of cell cultures 8. Interactions of animal viruses with cells, detection of virus replication 9. Monoclonal antibody technology (theory) 10. Immunization of laboratory mouse, culture of myeloma cells, preparation of feeder cells 11. Fusion of splenic lymphocytes with myeloma cells and cultivation in the selection medium 12. Cloning and storage of hybridomas 13. Production of monoclonal antibodies in vivo and in vitro.

Learning activities and teaching methods
Demonstration, Laboratory
  • Preparation for classes - 30 hours per semester
  • Class attendance - 30 hours per semester
Learning outcomes
To teach students the practical basics of working with cell cultures of animal origin.
Student will learn how to work with animal cell cultures (preparation of growth medium, cell passage, cell counting, cryopreservation, preparation of monoclonal antibodies).
Prerequisites
Basic laboratory skills. The subject introduces into the given issue, it does not require specific input knowledge.

Assessment methods and criteria
Student performance assessment, Systematic student observation

Students should learn the basics of working with cell cultures including sterile work, preparation of culture media, cell passages and storage. At least 80% attendance is required for obtaining the credit.
Recommended literature
  • Gibco, Invitrogen. CELL CULTURE BASICS, a handbook.
  • Helgason, Cheryl D., Miller, Cindy L. Basic Cell Culture Protocols. 2013. ISBN 978-1-62703-127-1.
  • J. Davis, ed. Basic Cell Culture: A Practical Approach, 2nd ed.. Oxford University Press, 2002. ISBN 9780199638536.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Agriculture and Technology Study plan (Version): Agricultural Biotechnology (2014) Category: Agriculture and forestry 2 Recommended year of study:2, Recommended semester: Winter