Lecturer(s)
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Kopecký Jan, prof. RNDr. CSc.
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Výletová Eva, Ing.
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Course content
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1. Types of tissue cultures, cell growth parameters 2. Nutritional requirements, culture media 3. Tissue culture laboratory equipment 4. Primary culture of guinea pig kidney 5. Cell lines, passaging and culture conditions 6. Cell cryopreservation 7. Contamination of cell cultures 8. Interactions of animal viruses with cells, detection of virus replication 9. Monoclonal antibody technology (theory) 10. Immunization of laboratory mouse, culture of myeloma cells, preparation of feeder cells 11. Fusion of splenic lymphocytes with myeloma cells and cultivation in the selection medium 12. Cloning and storage of hybridomas 13. Production of monoclonal antibodies in vivo and in vitro.
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Learning activities and teaching methods
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Demonstration, Laboratory
- Preparation for classes
- 30 hours per semester
- Class attendance
- 30 hours per semester
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Learning outcomes
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To teach students the practical basics of working with cell cultures of animal origin.
Student will learn how to work with animal cell cultures (preparation of growth medium, cell passage, cell counting, cryopreservation, preparation of monoclonal antibodies).
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Prerequisites
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Basic laboratory skills. The subject introduces into the given issue, it does not require specific input knowledge.
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Assessment methods and criteria
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Student performance assessment, Systematic student observation
Students should learn the basics of working with cell cultures including sterile work, preparation of culture media, cell passages and storage. At least 80% attendance is required for obtaining the credit.
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Recommended literature
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Gibco, Invitrogen. CELL CULTURE BASICS, a handbook.
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Helgason, Cheryl D., Miller, Cindy L. Basic Cell Culture Protocols. 2013. ISBN 978-1-62703-127-1.
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J. Davis, ed. Basic Cell Culture: A Practical Approach, 2nd ed.. Oxford University Press, 2002. ISBN 9780199638536.
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