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Lecturer(s)
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Kopecký Jan, prof. RNDr. CSc.
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Kovaříková Anna, Mgr.
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Course content
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1. Types of tissue cultures, cell growth parameters 2. Nutritional requirements, culture media 3. Tissue culture laboratory equipment 4. Primary culture of guinea pig kidney 5. Cell lines, passaging and culture conditions 6. Cell cryopreservation 7. Contamination of cell cultures 8. Interactions of animal viruses with cells, detection of virus replication 9. Monoclonal antibody technology (theory) 10. Immunization of laboratory mouse, culture of myeloma cells, preparation of feeder cells 11. Fusion of splenic lymphocytes with myeloma cells and cultivation in the selection medium 12. Cloning and storage of hybridomas 13. Production of monoclonal antibodies in vivo and in vitro.
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Learning activities and teaching methods
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Monitoring, Laboratory
- Class attendance
- 30 hours per semester
- Preparation for classes
- 30 hours per semester
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Learning outcomes
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To teach students the practical basics of working with cell cultures of animal origin
Ability to work with cell cultures (media preparation, passage of cells, cell counting, sterility testing, freezing of cells, monoclonal antibody technology).
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Prerequisites
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Basic skill in the laboratory. The subject introduces into the given issue, it does not require specific input knowledge.
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Assessment methods and criteria
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Systematic student observation, Interim evaluation
At least 80% attendance is required for obtaining the credit.
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Recommended literature
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J. Davis, ed.: Basic Cell Culture: A Practical Approach, 2nd ed., Oxford University Press, 2002, 416 pp..
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Gibco, Invitrogen. CELL CULTURE BASICS, a handbook.
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Helgason, Cheryl D., Miller, Cindy L. Basic Cell Culture Protocols. Humana Press, 2013. ISBN 978-1-62703-127-1.
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