| Course title | Cell Line Cultures in Vitro |
|---|---|
| Course code | KME/615 |
| Organizational form of instruction | Lecture + Lesson |
| Level of course | Master |
| Year of study | not specified |
| Frequency of the course | In each academic year, in the summer semester. |
| Semester | Summer |
| Number of ECTS credits | 3 |
| Language of instruction | English |
| Status of course | Compulsory-optional |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Course availability | The course is available to visiting students |
| Lecturer(s) |
|---|
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| Course content |
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1. Types of tissue cultures, cell growth parameters 2. Nutritional requirements, culture media 3. Tissue culture laboratory equipment 4. Primary culture of guinea pig kidney 5. Cell lines, passaging and culture conditions 6. Cell cryopreservation 7. Contamination of cell cultures 8. Interactions of animal viruses with cells, detection of virus replication 9. Monoclonal antibody technology (theory) 10. Immunization of laboratory mouse, culture of myeloma cells, preparation of feeder cells 11. Fusion of splenic lymphocytes with myeloma cells and cultivation in the selection medium 12. Cloning and storage of hybridomas 13. Production of monoclonal antibodies in vivo and in vitro.
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| Learning activities and teaching methods |
Monitoring, Laboratory
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| Learning outcomes |
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To teach students the practical basics of working with cell cultures of animal origin
Ability to work with cell cultures (media preparation, passage of cells, cell counting, sterility testing, freezing of cells, monoclonal antibody technology). |
| Prerequisites |
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Basic skill in the laboratory. The subject introduces into the given issue, it does not require specific input knowledge.
|
| Assessment methods and criteria |
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Systematic student observation, Interim evaluation
At least 80% attendance is required for obtaining the credit. |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Science | Study plan (Version): Infection Biology (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Infection Biology (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Molecular and Cell Biology and Genetics (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Molecular and Cell Biology and Genetics (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Molecular and Cell Biology and Genetics (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Infection Biology (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Molecular and Cell Biology and Genetics (1) | Category: Biology courses | - | Recommended year of study:-, Recommended semester: Summer |