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Lecturer(s)
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Piálková Radka, RNDr. Ph.D.
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Piálek Lubomír, RNDr. Ing. Ph.D.
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Course content
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Content of lectures: The focus of this course is on practical exercises that will be supplemented by specialized lectures on each topic. Content of practicals: 1. Collecting, fixation and preservation of genetic samples. Invasive and non-invasive methods. 2. DNA extraction, quality and quantity assessment. Quantitation of DNA by a fluorometric method. Agarose gel electrophoresis. 3. PCR amplification of standard phylogenetic/microsatellite markers. 4. Purification and separation techniques. Sequencing reaction preparation. 5. PCR as an analytical method. Allele-specific amplification - molecular sexing of birds, analysis of hybridism in cyprinid fishes. 6. Barcoding and its utility. Genealogical mtDNA test. 7. Introduction into processing of obtained sequence/microsatellite data.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Laboratory
- Class attendance
- 56 hours per semester
- Preparation for classes
- 4 hours per semester
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Learning outcomes
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The objective of the course is familiarization with methods of molecular biology applied in current zoological research.
Students acquire skills in basic molecular-biology laboratory techniques such as genomic DNA extraction, measuring of DNA concentration, quality assessment on agarose gel or PCR amplification in a context of methods used in current zoological research (sequencing of standard phylogenetic markers, barcoding, allele-specific amplification, microsatellite genotyping).
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Prerequisites
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No requirements on previous laboratory experience.
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Assessment methods and criteria
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Systematic student observation
100% presence and active participation in exercises is required.
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Recommended literature
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Šmarda J. a kol. Metody molekulární biologie. Masarykova univerzita, Brno, 2010..
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Šmehilová M. a kol. Laboratorní cvičení z molekulární biologie. Univerzita Palackého, Olomouc, 2014..
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Zima J. a kol. Genetické metody v zoologii. PřF UK, Praha, 2004..
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