Course: Enzymology

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Course title Enzymology
Course code UCH/021
Organizational form of instruction Lecture
Level of course Master
Year of study not specified
Frequency of the course In each academic year, in the winter semester.
Semester Winter
Number of ECTS credits 3
Language of instruction English
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Course availability The course is available to visiting students
Lecturer(s)
  • Komenda Josef, prof. RNDr. CSc., DSc.
  • Masojídek Jiří, prof. RNDr. CSc.
Course content
Enzyme definition, historical overview Cofactors, enzyme nomenclature, databases Getting enzymes: purification versus artificial synthesis Thermodynamic aspects of enzyme catalysis Enzyme kinetics The structure, folding and assembly of enzymes Localization and compartmentalization of enzymes Catalytic mechanisms of the enzyme action Regulation of enzyme activity Enzymes in biotechnology Structure-functional relationship in proteases Multifunctional and multienzyme complexes Clinical aspects of enzymology

Learning activities and teaching methods
Monologic (reading, lecture, briefing), Projection
  • Preparation for classes - 30 hours per semester
  • Class attendance - 26 hours per semester
  • Preparation for exam - 34 hours per semester
Learning outcomes
The course is supposed to provide a broadened knowledge on enzymes as the key catalytic and regulatory molecules of the cellular metabolism. The series of lectures will deal with the terminology and classification of enzymes, kinetic and thermodynamic aspects of their action, regulation of their activities in the cell (inhibitors and activators), mechanisms of their action and relationship between the structure and function, methods of their isolation and characterization and their utilization in the basic, clinical and applied research and in industry.
Student is familiar with the terminology and classification of enzymes, kinetic and thermodynamic aspects of their action, relationship between the structure and function, regulation of their activities in the cell and their utilization in the basic, clinical and applied research and in industry
Prerequisites
knowledge of general chemistry, organic chemistry and biochemistry

Assessment methods and criteria
Combined exam

attendance of the lectures, maximal 20% absence
Recommended literature
  • Jeremy M. Berg, John L. Tymoczko, Lubert Stryer : Biochemistry, The 5th edition, W. H. Freeman and Company New.
  • Nicholas C. Price, Lewis Stevens: Fundamentals of enzymology. The cell and molecular biology of catalytic proteins,.
  • Komenda, Masojídek. powerpoint presentation.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Science Study plan (Version): Biological chemistry (1) Category: Chemistry courses 2 Recommended year of study:2, Recommended semester: Winter