|
Lecturer(s)
|
-
Komenda Josef, prof. RNDr. CSc., DSc.
-
Masojídek Jiří, prof. RNDr. CSc.
|
|
Course content
|
Enzyme definition, historical overview Cofactors, enzyme nomenclature, databases Getting enzymes: purification versus artificial synthesis Thermodynamic aspects of enzyme catalysis Enzyme kinetics The structure, folding and assembly of enzymes Localization and compartmentalization of enzymes Catalytic mechanisms of the enzyme action Regulation of enzyme activity Enzymes in biotechnology Structure-functional relationship in proteases Multifunctional and multienzyme complexes Clinical aspects of enzymology
|
|
Learning activities and teaching methods
|
Monologic (reading, lecture, briefing), Projection
- Preparation for classes
- 30 hours per semester
- Class attendance
- 26 hours per semester
- Preparation for exam
- 34 hours per semester
|
|
Learning outcomes
|
The course is supposed to provide a broadened knowledge on enzymes as the key catalytic and regulatory molecules of the cellular metabolism. The series of lectures will deal with the terminology and classification of enzymes, kinetic and thermodynamic aspects of their action, regulation of their activities in the cell (inhibitors and activators), mechanisms of their action and relationship between the structure and function, methods of their isolation and characterization and their utilization in the basic, clinical and applied research and in industry.
Student is familiar with the terminology and classification of enzymes, kinetic and thermodynamic aspects of their action, relationship between the structure and function, regulation of their activities in the cell and their utilization in the basic, clinical and applied research and in industry
|
|
Prerequisites
|
knowledge of general chemistry, organic chemistry and biochemistry
|
|
Assessment methods and criteria
|
Combined exam
Students must correctly answer at least 50 % of test questions.
|
|
Recommended literature
|
-
Jeremy M. Berg, John L. Tymoczko, Lubert Stryer : Biochemistry, The 5th edition, W. H. Freeman and Company New.
-
Nicholas C. Price, Lewis Stevens: Fundamentals of enzymology. The cell and molecular biology of catalytic proteins,.
-
Komenda, Masojídek. powerpoint presentation.
|