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Lecturer(s)
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Fessl Tomáš, Mgr. Ph.D.
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Sýkora Jan, Mgr. Ph.D.
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Amaro Mariana, Dr. Ph.D.
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Course content
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Content of the course: Absorption of light and electronic transitions. Principles of Fluorescence. Principles and Selected Applications of Steady-State and Time-Resolved Fluorescence. Confocal and Two-Photon Fluorescence Microscopy. Recent Developments of Fluorescence Probes for Biosiences. Selected Applications of Fluorescence Microscopy in Cell Biology . Fluorescence in Non-viral Gene Therapy. Photodynamic Diagnosis and Therapy. Diagnosis of Prion Diseases by Fluorescence Correlation Spectroscopy
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Work with text (with textbook, with book), Monitoring, Individual preparation for exam
- Class attendance
- 28 hours per semester
- Preparation for classes
- 20 hours per semester
- Preparation for exam
- 35 hours per semester
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Learning outcomes
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Principles of fluorescence spectroscopy and microscopy and their applications in biology and medicine
The course introduces the students to the timely applications of the fluorescence spectroscopy and microscopy in biosciences. Students know and understand these applications (k1. K2). The students will be able to design and suggest appropriate fluorescence experiments for the specific biological problem (k6).
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Prerequisites
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The students are to know basics of the optical spectroscopy and microscopy. Moreover, the overview of the structure of biomolecules (DNA, proteins, lipids) and biomolecular self-assemblies (biomembranes, protein associates) is beneficial.
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Assessment methods and criteria
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Oral examination
The exam is oral. Randomly chosen 2 topics out of 12 is discussed and the understanding of the student to the particular fluorescence approach determines the evaluation.
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Recommended literature
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Hof Martin, Hutterer R., Fidler V: Fluorescence Spectroscopy in Biology. pp. 290, Springer Verlag, Heidelberg, 2004 (ISBN 3-540-22338-x).
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Kubitschek U.: Fluorescence Microscopy: From Principles to Biological Applications, Wiley-Blackwell, 1st ed. 2013.
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Lakowitz J.R., Principles of Fluorescence Spectroscopy, Springer, corr. 5th ed., 2010.
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