| Course title | Fluorescence Spectroscopy in Biological Systems |
|---|---|
| Course code | UCH/033 |
| Organizational form of instruction | Lecture |
| Level of course | Bachelor |
| Year of study | not specified |
| Frequency of the course | In each academic year, in the summer semester. |
| Semester | Summer |
| Number of ECTS credits | 3 |
| Language of instruction | English |
| Status of course | Compulsory-optional, Optional |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Course availability | The course is available to visiting students |
| Lecturer(s) |
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| Course content |
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Content of the course: Absorption of light and electronic transitions. Principles of Fluorescence. Principles and Selected Applications of Steady-State and Time-Resolved Fluorescence. Confocal and Two-Photon Fluorescence Microscopy. Recent Developments of Fluorescence Probes for Biosiences. Selected Applications of Fluorescence Microscopy in Cell Biology . Fluorescence in Non-viral Gene Therapy. Photodynamic Diagnosis and Therapy. Diagnosis of Prion Diseases by Fluorescence Correlation Spectroscopy
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| Learning activities and teaching methods |
Monologic (reading, lecture, briefing), Work with text (with textbook, with book), Monitoring, Individual preparation for exam
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| Learning outcomes |
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Principles of fluorescence spectroscopy and microscopy and their applications in biology and medicine
The course introduces the students to the timely applications of the fluorescence spectroscopy and microscopy in biosciences. The students will be able to design and suggest appropriate fluorescence experiments for the specific biological problem. |
| Prerequisites |
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The students are to know basics of the optical spectroscopy and microscopy. Moreover, the overview of the structure of biomolecules (DNA, proteins, lipids) and biomolecular self-assemblies (biomembranes, protein associates) is beneficial.
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| Assessment methods and criteria |
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Oral examination
The exam is oral. Randomly chosen 2 topics out of 12 is discussed and the understanding of the student to the particular fluorescence approach determines the evaluation. |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
|---|---|---|---|---|
| Faculty: Faculty of Science | Study plan (Version): Secondary Schools Teacher Training in Chemistry (1) | Category: Pedagogy, teacher training and social care | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Secondary Schools Teacher Training in Chemistry (1) | Category: Pedagogy, teacher training and social care | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Biophysics (1) | Category: Physics courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Biological chemistry (1) | Category: Chemistry courses | 2 | Recommended year of study:2, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Biological Chemistry (1) | Category: Chemistry courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Biophysics (1) | Category: Physics courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Biophysics (1) | Category: Physics courses | - | Recommended year of study:-, Recommended semester: Summer |
| Faculty: Faculty of Science | Study plan (Version): Biophysics (1) | Category: Physics courses | - | Recommended year of study:-, Recommended semester: Summer |