Course: Fluorescence Spectroscopy in Biological Systems

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Course title Fluorescence Spectroscopy in Biological Systems
Course code UCH/033
Organizational form of instruction Lecture
Level of course Bachelor
Year of study not specified
Frequency of the course In each academic year, in the summer semester.
Semester Summer
Number of ECTS credits 3
Language of instruction English
Status of course Compulsory-optional, Optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Course availability The course is available to visiting students
Lecturer(s)
  • Fessl Tomáš, Mgr. Ph.D.
  • Sýkora Jan, Mgr. Ph.D.
  • Amaro Mariana, Dr. Ph.D.
Course content
Content of the course: Absorption of light and electronic transitions. Principles of Fluorescence. Principles and Selected Applications of Steady-State and Time-Resolved Fluorescence. Confocal and Two-Photon Fluorescence Microscopy. Recent Developments of Fluorescence Probes for Biosiences. Selected Applications of Fluorescence Microscopy in Cell Biology . Fluorescence in Non-viral Gene Therapy. Photodynamic Diagnosis and Therapy. Diagnosis of Prion Diseases by Fluorescence Correlation Spectroscopy

Learning activities and teaching methods
Monologic (reading, lecture, briefing), Work with text (with textbook, with book), Monitoring, Individual preparation for exam
  • Class attendance - 28 hours per semester
  • Preparation for classes - 20 hours per semester
  • Preparation for exam - 35 hours per semester
Learning outcomes
Principles of fluorescence spectroscopy and microscopy and their applications in biology and medicine
The course introduces the students to the timely applications of the fluorescence spectroscopy and microscopy in biosciences. The students will be able to design and suggest appropriate fluorescence experiments for the specific biological problem.
Prerequisites
The students are to know basics of the optical spectroscopy and microscopy. Moreover, the overview of the structure of biomolecules (DNA, proteins, lipids) and biomolecular self-assemblies (biomembranes, protein associates) is beneficial.

Assessment methods and criteria
Oral examination

The exam is oral. Randomly chosen 2 topics out of 12 is discussed and the understanding of the student to the particular fluorescence approach determines the evaluation.
Recommended literature
  • Hof Martin, Hutterer R., Fidler V: Fluorescence Spectroscopy in Biology. pp. 290, Springer Verlag, Heidelberg, 2004 (ISBN 3-540-22338-x).
  • Kubitschek U.: Fluorescence Microscopy: From Principles to Biological Applications, Wiley-Blackwell, 1st ed. 2013.
  • Lakowitz J.R., Principles of Fluorescence Spectroscopy, Springer, corr. 5th ed., 2010.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Science Study plan (Version): Secondary Schools Teacher Training in Chemistry (1) Category: Pedagogy, teacher training and social care - Recommended year of study:-, Recommended semester: Summer
Faculty: Faculty of Science Study plan (Version): Secondary Schools Teacher Training in Chemistry (1) Category: Pedagogy, teacher training and social care - Recommended year of study:-, Recommended semester: Summer
Faculty: Faculty of Science Study plan (Version): Biophysics (1) Category: Physics courses - Recommended year of study:-, Recommended semester: Summer
Faculty: Faculty of Science Study plan (Version): Biological chemistry (1) Category: Chemistry courses 2 Recommended year of study:2, Recommended semester: Summer
Faculty: Faculty of Science Study plan (Version): Biological Chemistry (1) Category: Chemistry courses - Recommended year of study:-, Recommended semester: Summer
Faculty: Faculty of Science Study plan (Version): Biophysics (1) Category: Physics courses - Recommended year of study:-, Recommended semester: Summer
Faculty: Faculty of Science Study plan (Version): Biophysics (1) Category: Physics courses - Recommended year of study:-, Recommended semester: Summer
Faculty: Faculty of Science Study plan (Version): Biophysics (1) Category: Physics courses - Recommended year of study:-, Recommended semester: Summer