Course: Basic techniques in practical chemistry

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Course title Basic techniques in practical chemistry
Course code UCH/037
Organizational form of instruction Lesson
Level of course Bachelor
Year of study not specified
Frequency of the course In each academic year, in the winter semester.
Semester Winter
Number of ECTS credits 3
Language of instruction English
Status of course unspecified
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Course availability The course is available to visiting students
  • Klementová Šárka, doc. RNDr. CSc.
Course content
Content of practicals: 1.Fundamental safety precautions in chemical laboratory. 2.Report writing. 3.Graph construction and computer graphing. 4.Determining the mass, analytical and precission balance. 5.Preparing solutions of precise concentration. 6.Acid-base titration. 7.Filtration, gravity filtration (preparation and using of simple and fluted filter), suction filtration. 8.Centrifugation. 9.Potentiometric measurement of pH. 10.Spectrophototmetry - spectrophotometric calibration curve.

Learning activities and teaching methods
  • Preparation for classes - 15 hours per semester
  • Preparation for credit - 25 hours per semester
  • Class attendance - 35 hours per semester
Learning outcomes
Students master fundamental techniques used in chemical laboratories, such as preparing solutions of precise concentration, acid-base titration with indicator, gravity and suction filtration, centrifugation, potentiometric measurement of pH and determination of pK values of weak acids, spectrophotometric measurement - construction of spectrophotometric calibration curve and determination of concentration in unknown samples. Students will practise computer graph construction. English terminology necessary for detailed description of equipment and procedures is practised. The importance of keeping systematic laboratory notebook is emphasised; writing of final full-length reports is practised.
Students acquire skills in the very basic laboratory techniques used in chemical laboratory (weighing, filtration, vacuum filtration, centrifugation, preparing solutions of precise concentrations, acid-base titration with indicator; students also learn several instrumental methods such as potenciometry (pH measuring, usage of potentiometry titration for dissociation constant determination) and spectrophotometry (construction and usage spectrophotometric calibration curve). Students master the fundamentals in preparing final reports on experimental results of laboratory experiments including correct referring to sources of information.
No special knowledge or competences are expected for enrolment to the course.

Assessment methods and criteria
Analysis of student's work activities (technical works), Development of laboratory protocols

To pass the course, all laboratory experiments must be performed, reports of selected experiments must be completed.
Recommended literature
  • Basic Science Lab Equipment:
  • Potentiometric measurement of pH: (August, 2009).
  • Risk and safety: (August, 2005).
  • Safety in chemical laboratory - a practical guide for teachers..
  • Science Lab Techniques: (August, 2009).
  • Spectrophotometry: (August, 2009).
  • Texts provided by the coordinator..

Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester