Course: Laboratory Course in Biochemistry

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Course title Laboratory Course in Biochemistry
Course code UCH/049
Organizational form of instruction Lesson
Level of course Bachelor
Year of study not specified
Frequency of the course In each academic year, in the winter semester.
Semester Winter
Number of ECTS credits 4
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Mašková Hana, Mgr.
  • Hejduk Libor, Mgr.
  • Selinger Martin, RNDr. Ph.D.
  • Štěrba Ján, RNDr. Ph.D.
  • Kočová Pavlína, Mgr.
  • Havlíčková Petra, Bc.
  • Kozelková Tereza, Mgr.
Course content
Purification of proteins - precipitation with salts and organic solvents, dialysis followed by chromatographic purification. Chromatographic methods in Biochemistry - separation of proteins using gel chromatography, ionex chromatography, and affinity chromatography. DNA isolation and analysis - Isolation of plasmid DNA, chromosomal DNA, measurement of DNA concentration, measurement of DNA concentration in microvolumes, agarose gel electrophoresis of DNA. Methods for protein concentration determination - quantification of proteins using the Lowry method, biret method, Bradford method, direct determination of protein concentration, direct determination of protein concentration in microvolumes. Enzyme kinetics - kinetic parameters of protein cleavage by trypsin, kinetic parameters of hydrogen peroxide cleavage by catalase, kinetic paramteres of peroxidase reaction. SDS-PAGE of proteins - electrophoretic separation of proteins in polyacrylamide gels of different concentrations, staining of the seaprated proteins using the CBB dye.

Learning activities and teaching methods
Skills training, Laboratory
  • Class attendance - 60 hours per semester
  • Preparation for credit - 20 hours per semester
  • Preparation for classes - 20 hours per semester
Learning outcomes
Laboratory course introduces students to selected basic topics in analytical and preparative biochemistry.
Learning of the basic methods in biochemistry
Prerequisites
UCH/006
UCH/006

Assessment methods and criteria
Student performance assessment, Test

Active work in the laboratory, preparation for the topics, obtaining sufficient points in the tests.
Recommended literature
  • Káš a kol., VŠCHT. Laboratorní techniky biochemie.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Science Study plan (Version): Environmental chemistry (1) Category: Engineering chemistry and chemistry of silicates - Recommended year of study:-, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Chemistry for future teachers (1) Category: Chemistry courses 2 Recommended year of study:2, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Biophysics (1) Category: Physics courses 2 Recommended year of study:2, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Biomedical Laboratory Techniques (1) Category: Biology courses 2 Recommended year of study:2, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Chemistry for future teachers (1) Category: Chemistry courses 2 Recommended year of study:2, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Chemistry for future teachers (1) Category: Chemistry courses 2 Recommended year of study:2, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Secondary Schools Teacher Training in Chemistry (1) Category: Pedagogy, teacher training and social care - Recommended year of study:-, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Chemistry (1) Category: Chemistry courses 2 Recommended year of study:2, Recommended semester: Winter
Faculty: Faculty of Science Study plan (Version): Secondary Schools Teacher Training in Chemistry (1) Category: Pedagogy, teacher training and social care - Recommended year of study:-, Recommended semester: Winter