| Course title | Laboratory Course in Biochemistry |
|---|---|
| Course code | UCH/049 |
| Organizational form of instruction | Lesson |
| Level of course | Bachelor |
| Year of study | 2 |
| Frequency of the course | In each academic year, in the winter semester. |
| Semester | Winter |
| Number of ECTS credits | 4 |
| Language of instruction | Czech |
| Status of course | Compulsory |
| Form of instruction | Face-to-face |
| Work placements | This is not an internship |
| Recommended optional programme components | None |
| Lecturer(s) |
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| Course content |
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Purification of proteins - precipitation with salts and organic solvents, dialysis followed by chromatographic purification. Chromatographic methods in Biochemistry - separation of proteins using gel chromatography, ionex chromatography, and affinity chromatography. DNA isolation and analysis - Isolation of plasmid DNA, chromosomal DNA, measurement of DNA concentration, measurement of DNA concentration in microvolumes, agarose gel electrophoresis of DNA. Methods for protein concentration determination - quantification of proteins using the Lowry method, biuret method, Bradford method, direct determination of protein concentration, direct determination of protein concentration in microvolumes. Enzyme kinetics - kinetic parameters of protein cleavage by trypsin, kinetic parameters of hydrogen peroxide cleavage by catalase, kinetic parameters of peroxidase reaction. SDS-PAGE of proteins - electrophoretic separation of proteins in polyacrylamide gels of different concentrations, staining of the separated proteins using the CBB dye.
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| Learning activities and teaching methods |
Skills training, Laboratory
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| Learning outcomes |
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The laboratory focuses on the selected topics in analytical and preparative biochemistry while teaching basic techniques needed for work in the biochemistry laboratory.
Learning of the basic methods in biochemistry |
| Prerequisites |
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UCH/006
UCH/CV006 ----- or ----- UCH/006 |
| Assessment methods and criteria |
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Student performance assessment, Test
Active work in the laboratory, theoretical preparation for laboratory tasks (tested before laboratory entry with two retake options), 100% participation in introductory lecture and all laboratory tasks and work on the assignments (results calculation from the measurements), and obtaining 60% of points in the tests. |
| Recommended literature |
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| Study plans that include the course |
| Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester |
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