Course title | Laboratory Course in Biochemistry |
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Course code | UCH/049 |
Organizational form of instruction | Lesson |
Level of course | Bachelor |
Year of study | not specified |
Frequency of the course | In each academic year, in the winter semester. |
Semester | Winter |
Number of ECTS credits | 4 |
Language of instruction | Czech |
Status of course | Compulsory |
Form of instruction | Face-to-face |
Work placements | This is not an internship |
Recommended optional programme components | None |
Lecturer(s) |
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Course content |
Purification of proteins - precipitation with salts and organic solvents, dialysis followed by chromatographic purification. Chromatographic methods in Biochemistry - separation of proteins using gel chromatography, ionex chromatography, and affinity chromatography. DNA isolation and analysis - Isolation of plasmid DNA, chromosomal DNA, measurement of DNA concentration, measurement of DNA concentration in microvolumes, agarose gel electrophoresis of DNA. Methods for protein concentration determination - quantification of proteins using the Lowry method, biuret method, Bradford method, direct determination of protein concentration, direct determination of protein concentration in microvolumes. Enzyme kinetics - kinetic parameters of protein cleavage by trypsin, kinetic parameters of hydrogen peroxide cleavage by catalase, kinetic parameters of peroxidase reaction. SDS-PAGE of proteins - electrophoretic separation of proteins in polyacrylamide gels of different concentrations, staining of the separated proteins using the CBB dye.
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Learning activities and teaching methods |
Skills training, Laboratory
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Learning outcomes |
The laboratory focuses on the selected topics in analytical and preparative biochemistry while teaching basic techniques needed for work in the biochemistry laboratory.
Learning of the basic methods in biochemistry |
Prerequisites |
UCH/006
UCH/CV006 ----- or ----- UCH/006 |
Assessment methods and criteria |
Student performance assessment, Test
Active work in the laboratory, theoretical preparation for laboratory tasks (tested before laboratory), 100% participation in all laboratory tasks, and obtaining 60% of points in the tests. |
Recommended literature |
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Study plans that include the course |
Faculty | Study plan (Version) | Category of Branch/Specialization | Recommended semester | |
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Faculty: Faculty of Science | Study plan (Version): Secondary Schools Teacher Training in Chemistry (1) | Category: Pedagogy, teacher training and social care | - | Recommended year of study:-, Recommended semester: Winter |
Faculty: Faculty of Science | Study plan (Version): Chemistry (1) | Category: Chemistry courses | 2 | Recommended year of study:2, Recommended semester: Winter |
Faculty: Faculty of Science | Study plan (Version): Biophysics (1) | Category: Physics courses | 2 | Recommended year of study:2, Recommended semester: Winter |
Faculty: Faculty of Science | Study plan (Version): Chemistry for future teachers (1) | Category: Chemistry courses | 2 | Recommended year of study:2, Recommended semester: Winter |
Faculty: Faculty of Science | Study plan (Version): Biomedical Laboratory Techniques (1) | Category: Biology courses | 2 | Recommended year of study:2, Recommended semester: Winter |