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Lecturer(s)
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Koutská Anna, Mgr.
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Kaščáková Barbora, Mgr. Ph.D.
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Course content
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Chromatographic methods in Biochemistry - separation of proteins using gel chromatography, ionex chromatography, and affinity chromatography. DNA isolation and analysis - Isolation of plasmid DNA, chromosomal DNA, measurement of DNA concentration, measurement of DNA concentration in microvolumes, agarose gel electrophoresis of DNA. Methods for protein concentration determination - quantification of proteins using the Lowry method, biret method, Bradford method, direct determination of protein concentration, direct determination of protein concentration in microvolumes. Enzyme kinetics - kinetic parameters of protein cleavage by trypsin, kinetic parameters of hydrogen peroxide cleavage by catalase, kinetic paramteres of peroxidase reaction.
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Learning activities and teaching methods
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Skills training, Laboratory
- Preparation for classes
- 16 hours per semester
- Class attendance
- 32 hours per semester
- Preparation for credit
- 6 hours per semester
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Learning outcomes
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Laboratory course introduces students to selected basic topics in analytical and preparative biochemistry.
Lab. course for the students of the Czech programmes Biologie, Péče o životní prostředí.
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Prerequisites
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Students use the knowledge gained in the Biochemistry and other lectures in laboratory work.
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Assessment methods and criteria
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Student performance assessment, Test, Development of laboratory protocols
Active work in the laboratory, preparation for the topics, obtaining sufficient points in the tests.
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Recommended literature
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Káš a kol., VŠCHT. Laboratorní techniky biochemie.
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