Course: Biocrystallization methods

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Course title Biocrystallization methods
Course code UCH/253
Organizational form of instruction Lecture + Lesson
Level of course Master
Year of study not specified
Frequency of the course In each academic year, in the summer semester.
Semester Summer
Number of ECTS credits 8
Language of instruction Czech
Status of course Compulsory
Form of instruction unspecified
Work placements unspecified
Recommended optional programme components None
Lecturer(s)
  • Prudnikova Taťána, MSc. Ph.D.
  • Kutý Michal, Mgr. Ph.D.
  • Kutá Smatanová Ivana, prof. Mgr. Ph.D.
Course content
Content of lectures: Macromolecular substances - basic terms Crystallization - principles and methods of crystallization properties of solutions and phase diagrams automation in crystallization of proteins nucleation and crystal growth, growth kinetics membrane proteins crystallization standard, advanced and alternative crystallization methods - theory and applications in practice Content of exercises: Methods of protein crystallization, crystallization experiments with model proteins (eg crystallization of lysozyme, proteinase K, etc.), familiarization with the work of crystallization robots, preparation of protein crystals for measurement on a diffractometer, introduction to the structure solution of protein using PX software.

Learning activities and teaching methods
Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Work with text (with textbook, with book), Demonstration, Laboratory, Activating (simulations, games, drama)
  • Class attendance - 65 hours per semester
  • Preparation for credit - 10 hours per semester
  • Preparation for exam - 10 hours per semester
  • Preparation for classes - 10 hours per semester
Learning outcomes
The X-ray crystallography is becoming one of the most important techniques to study proteins, nucleic acids and their complexes as well as viruses and supramolecular complexes at an atomic resolution. Crystallization of biomacromolecules is an empirical process based on finding the individual conditions and parameters for growing crystals and their optimization. The limited condition of crystallization is growing crystals suitable for X-ray diffraction measurement. The course is aimed at familiarization of students with standard and advanced crystallization methods and techniques. During the lab exercises students will grow crystals of model or own proteins using of standard and advanced crystallization techniques.
Graduates will became familiar with methods and techniques of crystallisation of important biomolecular compounds like proteins and nucleic acids. They will be able to find by own individual conditions and parameters for growing crystals and their optimalization using standard, advanced and alternative crystallisation techniques, moreover they will know how to test character of crystals and prepare crystals for diffraction measurements on the X-ray sources (diffractometers and synchrotrons) for a final determination of macromolecular structure.
Prerequisites
Completion of a general and organic chemistry and biochemistry courses and laboratory work.

Assessment methods and criteria
Student performance assessment, Combined exam, Development of laboratory protocols

Requirement for successful completion of the course is the active participation of the student in lectures and practical exercises. The final exam will take the form of a written test and a subsequent oral interview with a final mark in the form of a mark.
Recommended literature
  • A.Ducruix; R. Giege. Crystallization of nucleic acids and proteins : a practical approach 2Ed. Oxford University Press, 2000. ISBN 9780199636785.
  • Daniel M. Bollag, Michael D. Rozycki, Stuart J. Edelstein. Protein Methods, 2nd Edition. Wiley, 1996. ISBN 978-0-471-11837-4.
  • Jan Drenth. Principles of Protein X-Ray Crystallography 2Ed. Springer Verlag Publishing, 2002. ISBN 978-0-387-33746-3.
  • T.M. Bergfors. Protein Crystallization. Techniques, Strategies, and Tips. International University Line, 1999. ISBN 0963681753.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Science Study plan (Version): Biophysics (1) Category: Physics courses - Recommended year of study:-, Recommended semester: Summer