UNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE
Study programmes & Course catalogue
for academic year 2025/2026
UNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE
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Course: Instrumental methods of environmental monitoring - practical 2

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Course title Instrumental methods of environmental monitoring - practical 2
Course code UCH/549
Organizational form of instruction Lesson
Level of course Master
Year of study not specified
Frequency of the course In academic years starting with an odd year (e.g. 2019/2020), in the winter semester.
Semester Winter
Number of ECTS credits 5
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Kahoun David, Ing. Ph.D.
  • Hořejší Karel, Mgr. Ph.D.
Course content
Content of laboratory excercises: Exercise No. 1: Determination of polychlorinated biphenyls (PCBs) in water after liquid-liquid extraction (LLE) using gas chromatography with electron capture detection (GC-MS/MS). Exercise No. 2: Determination of triazine pesticides in water after solid phase extraction (SPE) using gas chromatography with tandem mass spectrometry detection (GC-MS/MS). Exercise No. 3: Determination of polycyclic aromatic hydrocarbons (PAHs) in soil after solid-liquid extraction (SLE) using high-performance liquid chromatography with spectrophotometric and fluorescence detection (LC-UV/VIS-FLD). Exercise No. 4: Determination of antibiotic ciprofloxacin in water using high-performance liquid chromatography with tandem mass spectrometry detection (LC-MS/MS).

Learning activities and teaching methods
Demonstration, Laboratory
  • Class attendance - 40 hours per semester
  • Preparation for classes - 10 hours per semester
  • Preparation for credit - 64 hours per semester
  • Preparation for exam - 0 hours per semester
Learning outcomes
To acquire practical knowledge and skills in the use of advanced instrumental analytical methods.
Student will acquire knowledge of using of advanced instrumental analytical methods.
Prerequisites
Basic knowledge of principles of gas chromatography, liquid chromatography, spectrophotometric detection, fluorescence detection and mass detection (target analysis). Good knowlegde of chemical nomenclature and chemical calculations.

Assessment methods and criteria
Development of laboratory protocols

To pass the course student must perform all laboratory exercises and hand in all laboratory protocols worked out in sufficient quality.
Recommended literature
  • 4. Churáček, Jaroslav, a další. Analytická separace látek. Praha : SNTL - nakladatelství technické literatury, 1990. 80-03-00569-8..
  • Christian, Gary D.; Dasgupta, Purnendu K.; Schug, Kevin. Analytical chemistry. 7th ed. Hoboken, N.J. : Wiley, 2014. ISBN 978-0-470-88757-8.
  • Křížek, Martin; Šíma, Jan. Analytická chemie. 1. vydání. České Budějovice : Jihočeská univerzita v Českých Budějovicích, Zemědělská fakulta, 2015. ISBN 978-80-7394-486-5.
  • Nováková, Lucie; Douša, Michal,; Česla, Petr. Moderní HPLC separace v teorii a praxi. I.. 2. přepracované a rozšířené vydání. Brno : Česká chromatografická škola, zapsaný spolek, 2021. ISBN 978-80-270-8559-0.
  • Nováková, Lucie; Douša, Michal. Moderní HPLC separace v teorii a praxi. II.. 2. upravené vydání. Brno : Česká chromatografická škola, zapsaný spolek, 2021. ISBN 978-80-270-8560-6.
  • Skoog, Douglas A.; West, Donald M.; Holler, F. James; Crouch, Stanley R.,; Nesměrák, Karel,; Červený, Václav,; Křížek, Tomáš,; Nováková, Eliška. Analytická chemie. Vydání: první. Praha : Vysoká škola chemicko-technologická v Praze, 2019. ISBN 978-80-7592-043-0.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
UNIVERSITY OF SOUTH BOHEMIA IN ČESKÉ BUDĚJOVICE, date of update: 30.10.2025 23:50. Data created for academic year 2025/2026