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Lecturer(s)
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Vácha František, prof. RNDr. Ph.D.
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Course content
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Introductory metabolism and bioenergetics: Metabolism; Thermodynamics of energetic metabolism; Biological oxidation; Coupled reactions; Experimental approach. Energetic metabolism I: Carbohydrate metabolism; Compartmentation; Regulation. Energetic metabolism II: The Citric acid cycle; Electron transport; Oxidative phosphorylation. Energetic metabolism III: Lipid metabolism; Nitrogen utilization; Compartmentation. Energetic metabolism IV: Photosynthesis and photosynthetic machinery; Photorespiration; the C4 cycle. Integration and regulation of energetic metabolism: Compartmentation of metabolic pathways; Functinal relatioships between pathways; Regulatory actions; Metabolic check points; Experimental approaches in metabolism. Contents of laboratories: 1.Isolation of nucleic acids from algae. 2. Affinity chromatography of photosystem II.. 3. Separation of amino acids by ion exchange. 4. Cleavage of gelatine by trypsin, Michaelis constant determination. 5. Electrophoresis of proteins from thylacoid membranes. 6. Determination of relative molecular weight of peroxidase by gel filtration. 7. Kinetics of Hill`s reaction. 8. Affinity chromatography of IgG. 9. Isolation of lectins from the seesd of leguminous plants. 10. Analysis of carbohydrates by anthrone agent. 11. Isolation of horse radish peroxidase. 12. Preparation of thylacoid membrane fractions on density gradient of succrose. 13. Spectrophotometry determination of tyrosine and tryptophane in proteins. 14. Quantitative analysis of glucose. 15. Time course analysis of cleavage of gelatine by trypsin. 16. Measurement of peroxidase activity: estimation of kinetic parameters. 17. Isolation of mannan from yeast. 18. Glycans of cellular glycoproteins: analysis by affiinity blotting. 19. Imunoblotting of tick heamolymph with rabbit antibodies against lectin. 20. Isolation of DNA from calf thymus. 21. Isolation of DNA from liver of laboratory rat.
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming)
- Class attendance
- 52 hours per semester
- Preparation for exam
- 50 hours per semester
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Learning outcomes
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Course aims at introduction of general biochemistry for students of the Biological Chemistry bachelor study programme. The course provides a broad array of fundamental knowledge of structural and functional biochemistry which are essential for understanding of other subjects in Biological Chemistry curriculum. In the course framework, biochemistry is given as chemistry of non-binding/non-covalent interactions which take place in biological systems on the level of molecules and cells. Structural and functional properties of the components which play a role in the transmission of energy as well as signals are emphasized.
Students will gain knowledge on the basics of biochemistry and must pass a test.
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Prerequisites
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Biochemistry I (UCH/757), knowledge of general, inorganic, organic chemistry and cell biology.
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Assessment methods and criteria
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Test
Students must correctly answer at least 50 % of test questions.
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Recommended literature
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A. L. Lehninger, D. L. Nelson, M. M. Cox: Principles of Biochemistry. Worth Publishers, New York, 1993.
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D. Voet, J. G. Voet: Biochemistry. Victoria Publishing Ltd.
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J. Stenesh: Biochemistry. Plenum Press,New York&London, 1998.
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