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Lecturer(s)
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Havlíčková Petra, RNDr.
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Kaftan David, Mgr. Ph.D.
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Dvorníková Zuzana, Mgr.
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Jaklová Kateřina, Mgr.
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Doudová Lenka, Mgr.
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Frejlachová Andrea, Mgr.
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Tučková Dominika, Ing.
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Selinger Martin, RNDr. Ph.D.
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Mašková Hana, Mgr.
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Hejduk Libor, Mgr.
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Tykalová Hana, Mgr. Ph.D.
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Course content
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Purification of proteins - precipitation with salts and organic solvents, dialysis followed by chromatographic purification. Chromatographic methods in Biochemistry - separation of proteins using one of the selected chromatographic methods, such as gel chromatography, ionex chromatography, or affinity chromatography. Methods for protein concentration determination - quantification of proteins using the Lowry method, biuret method, Bradford method, direct determination of protein concentration, direct determination of protein concentration in microvolumes. Enzyme kinetics - kinetic parameters of protein cleavage by trypsin, kinetic parameters of hydrogen peroxide cleavage by catalase, kinetic parameters of peroxidase reaction. SDS-PAGE of proteins - electrophoretic separation of proteins in polyacrylamide gels of different concentrations, staining of the separated proteins using the CBB dye.
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Learning activities and teaching methods
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Skills training, Laboratory
- Class attendance
- 42 hours per semester
- Preparation for credit
- 8 hours per semester
- Preparation for classes
- 8 hours per semester
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Learning outcomes
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The laboratory course focuses on the selected topics in analytical and preparative biochemistry while teaching basic techniques needed for work in the biochemistry laboratory.
Students know and understand the principles of the basic biochemical methods (k1, k2), can perform such experiments (k3), analyze the results (k4), and draw conclusions (k5). Students can apply this knowledge and design experiments for needed analyses (k6).
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Prerequisites
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Povinná prerekvizita pro studenty Biological Chemistry: Laboratory Course in General Chemistry (JKU, Linz); or the courses Introductory Lab Course and the Lab Course in Analytical Chemistry.
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Assessment methods and criteria
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Student performance assessment, Test
Active work in the laboratory, theoretical preparation for laboratory tasks (tested before laboratory entry with two retake options), 100% participation in all laboratory tasks and work on the assignments (results calculation from the measurements), and obtaining 60% of points in the tests.
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Recommended literature
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Štěrba et al. Manual - Biochemistry Laboratory. 2024.
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