Course: Experimental Methods - Laboratory Exercises

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Course title Experimental Methods - Laboratory Exercises
Course code UCH/959
Organizational form of instruction Lesson
Level of course Master
Year of study not specified
Frequency of the course In each academic year, in the summer semester.
Semester Summer
Number of ECTS credits 4
Language of instruction Czech
Status of course unspecified
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Litvín Radek, RNDr. Ph.D.
  • Bína David, RNDr. Ph.D.
  • Věchtová Pavlína, RNDr. Ph.D.
  • Kutá Smatanová Ivana, prof. Mgr. Ph.D.
  • Kahoun David, Ing. Ph.D.
  • Štěrba Ján, RNDr. Ph.D.
  • Prudnikova Taťána, MSc. Ph.D.
  • Fessl Tomáš, Mgr. Ph.D.
  • Koník Peter, Mgr.
  • Ďurinová Eva, Mgr.
Course content
Content of practicals: Each student will draw up its own scientific mini-project, in which he/she will test and characterize own sample using mentioned methods. At the end, student will write a protocol, which will assess the results and evaluate the advantages and disadvantages of different experimental methods using in the project. 1. Purification of peroxidase from horseradish by ammonium sulfate precipitation and ion exchange chromatography using TEAE Spheron 2. RNA isolation 3. Determination of peroxidase gene expression 4. Protein identification using LC-MS/MS 5. Crystallization experiment 6. Qualitative analysis of amino acids by method of high performance liquid chromatography with ionization heated by electrospray and tandem mass detection (HPLC-HESI-MS / MS) 7. Use of spectral methods to characterize sample - Circular Dichroism 8. Electron microscopy 9. Evaluation Results 10. Project processing and presentation

Learning activities and teaching methods
Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Work with text (with textbook, with book), Demonstration, Excursion
  • Semestral paper - 28 hours per semester
  • Class attendance - 28 hours per semester
Learning outcomes
Laboratory exercises will focus on the practical application of methods which have been separately discussed during lectures given in first and second semester of Master's degree program.
Course graduates are practically acquainted with experimental methods used in structural and functional studies of proteins and macromolecular complexes from sample preparation through testing to structural studies.
The minimum requirement for students is knowledge of general and organic chemistry and biochemistry and the basics of laboratory practice.

Assessment methods and criteria
Interview, Seminar work

Student gets credit after submission of laboratory protocol and subsequent consultation of obtained results.
Recommended literature
  • Viz sylaby jednotlivých předmětů. / See course characteristics of individual subjects..

Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester