|
Lecturer(s)
|
-
Kutá Smatanová Ivana, prof. Mgr. Ph.D.
-
Prudnikova Taťána, MSc. Ph.D.
|
|
Course content
|
Content of practicals: Each student will draw up its own scientific mini-project, in which he/she will test and characterize own sample using mentioned methods. At the end, student will write a protocol, which will assess the results and evaluate the advantages and disadvantages of different experimental methods using in the project. 1. Purification of peroxidase from horseradish by ammonium sulfate precipitation and ion exchange chromatography using TEAE Spheron 2. RNA isolation 3. Determination of peroxidase gene expression 4. Protein identification using LC-MS/MS 5. Crystallization experiment 6. Qualitative analysis of amino acids by method of high performance liquid chromatography with ionization heated by electrospray and tandem mass detection (HPLC-HESI-MS / MS) 7. Use of spectral methods to characterize sample - Circular Dichroism 8. Electron microscopy 9. Evaluation Results 10. Project processing and presentation
|
|
Learning activities and teaching methods
|
Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Work with text (with textbook, with book), Demonstration, Excursion
- Semestral paper
- 28 hours per semester
- Class attendance
- 28 hours per semester
|
|
Learning outcomes
|
Laboratory exercises will focus on the practical application of methods which have been separately discussed during lectures given in first and second semester of Master's degree program.
Course graduates are practically acquainted with experimental methods used in structural and functional studies of proteins and macromolecular complexes from sample preparation through testing to structural studies.
|
|
Prerequisites
|
The minimum requirement for students is knowledge of general and organic chemistry and biochemistry and the basics of laboratory practice.
|
|
Assessment methods and criteria
|
Interview, Seminar work
Student gets credit after submission of laboratory protocols and subsequent consultation of obtained results.
|
|
Recommended literature
|
-
Viz sylaby jednotlivých předmětů. / See course characteristics of individual subjects..
|