Course: Glycobiochemistry

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Course title Glycobiochemistry
Course code UCH/961
Organizational form of instruction Lecture
Level of course Master
Year of study not specified
Frequency of the course In each academic year, in the winter semester.
Semester Winter
Number of ECTS credits 3
Language of instruction Czech
Status of course unspecified
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Štěrba Ján, RNDr. Ph.D.
  • Grubhoffer Libor, prof. RNDr. CSc.
  • Štěrbová Jarmila, RNDr.
  • Věchtová Pavlína, RNDr. Ph.D.
Course content
Content of lectures: 1. Posttranslational modifications of proteins. Introduction to glycobiology. Glycans, glycoproteins, lectins, and their importance. 2. Glycosylation. N-glycans, their importance in proteosynthesis. 3. O-glycans, other glycan types. 4. N-glycan biosynthesis in Eukaryotes and bacteria, its localization in the cell. 5. Glycosyltransferases and glycosidases. 6. Differences in glycosylation - glycosylation in mammals, arthropods, plants, parasites, and bacteria. 7. Clinical aspects of glycosylation, sialic acid, fucose, galactose - glycans and infection, inherited glycosylation disorders, allergic reactions, transplantation. 8. Clinical aspects of glycosylation, sialic acid, fucose, galactose - allergic reactions, glycans as disease markers. 9. Lectins and saccharide/glycans-specific antibodies. Galectins, C-lectins, P-lectins, etc. 10. Glycomics, glycoproteomics - the use of lectins and antibodies. Structural analysis, chemical and enzymatic synthesis. Glycosylation inhibitors. Glycan-labelling approaches. 11. Glycomics, glycoproteomics - chemical and enzymatic synthesis. Glycosylation inhibitors. Glycan-labelling approaches. 12. Glycobiology in biotechnology - production of recombinant glycoproteins and problems related to their use. Examples.

Learning activities and teaching methods
Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming)
  • Class attendance - 30 hours per semester
  • Preparation for classes - 25 hours per semester
  • Preparation for exam - 20 hours per semester
Learning outcomes
Lectures will introduce the students to the world of posttranlastional modifications of proteins, primarily glycosylation. The different glycosylation types will be explained as well as their biosynthesis, differences in glycan structures between various organisms and importance of these differences from the medicinal point of view. Lastly, methods for glycans and glycoproteins analyses will be shown.
Basic knowledge in glycobiology and glycobiochemistry will be provided
Prerequisites
Biochemistry, Mol. and Cellular Biology

Assessment methods and criteria
Interview, Combined exam

The student must pass an oral exam.
Recommended literature
  • Essentials of Glycobiology, 2nd edition, Editors: Ajit Varki, Richard D. Cummings, Jeffrey D. Esko, Hudson H. Freeze, Pamela Stanley, Carolyn R. Bertozzi, Gerald W. Hart, Marilynn E. Etzler. Cold Spring Harbor Laboratory Press; 2009..
  • Glycobiology, Clare Sansom, Ofer Markoman, Scion Publishing, 2007.
  • The Sugar Code, Editor: Hans-Joachim Gabius, Wiley-Blackwell, 2009.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester