Course: Xenobiochemistry

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Course title Xenobiochemistry
Course code UCH/962
Organizational form of instruction Lecture + Lesson
Level of course Master
Year of study not specified
Frequency of the course In each academic year, in the winter semester.
Semester Winter
Number of ECTS credits 3
Language of instruction Czech
Status of course unspecified
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Štěrba Ján, RNDr. Ph.D.
  • Věchtová Pavlína, RNDr. Ph.D.
Course content
Content of lectures: 1. Introduction, definition of xenobiotic and xenobiochemistry, history, the fate of xenobiotics in the organism, transport, distribution, accumulation, metabolism and elimination. 2. I., II., and III. Phase of biotransformation, reactions and enzymes. 3. Cytochromes P450, FMO and other oxidative enzymes. 4. Reductive enzymes. 5. Reactions of II. phase of biotransformation. 6. Reactions of II. phase of biotransformation. 7. Factors influencing biotransformation. 8. Activation and inactivation of biotransformation enzymes (Ah receptors and other nuclear receptors). 9. Biotransformation in organs, organ toxicity. 10. Biotransformation in organs, organ toxicity. 11. Biotransformation in the foetus and placenta, toxicity and accumulation of xenobiotics in the foetus. 12. Examples of biotransformation of specific xenobiotics (drugs etc.). Ecotoxicology. Content of practicals: DNA damage detection, methods for detection of mutations, determination of activity of biotransforming enzymes, determination of mutations affecting the activity of biotransforming enzymes (e.g. CYP2D6, ADH, ALDH). Analytical methods in toxicology - LC-MS, GC-MS etc.

Learning activities and teaching methods
Monologic (reading, lecture, briefing), Laboratory, Activating (simulations, games, drama)
  • Preparation for classes - 18 hours per semester
  • Preparation for exam - 15 hours per semester
  • Class attendance - 52 hours per semester
Learning outcomes
The fate of xenobiotics will be introduced to the students, xenobiotics metabolism, and the metabolic pathways.
Student learns the basics of xenobiochemical processes
Prerequisites
Biochemistry , Cell biology, Molecular biology

Assessment methods and criteria
Essay, Student performance assessment, Combined exam, Test, Interim evaluation

The student must actively participate in the practical part, prepare an essay on a selected topic, and pass the test.
Recommended literature
  • Casarett & Doull's Essentials of Toxicology, 2nd Ed., Curtis D. Klaassen, John B. Watkins III, McGraw-Hill 2010.
  • Molecular Toxicology, 2nd Ed., P. David Josephy, Bengt Mannervik, Oxford University Press, 2006.
  • The Chemistry and Metabolism of Drugs and Toxins: An Introduction to Xenobiochemistry, Michael Briggs, Maxine Briggs, Elsevier 1974.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester