Course: Optical Methods in Biochemistry

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Course title Optical Methods in Biochemistry
Course code UCH/965
Organizational form of instruction Lecture + Lesson
Level of course Master
Year of study not specified
Frequency of the course In each academic year, in the summer semester.
Semester Summer
Number of ECTS credits 3
Language of instruction Czech
Status of course unspecified
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Kopecký Vladimír, prof. RNDr. Ph.D.
  • Litvín Radek, RNDr. Ph.D.
  • Bína David, RNDr. Ph.D.
Course content
Content of lectures: 1. Theoretical background: electromagnetic radiation, interaction of light with matter, absorption, emission, dispersion 2. Practical aspects: radiation sources, detection methods; principles, and use 3. Methods based on dispersion of light by macromolecules, DLS; absorption spectroscopy part I 4. Absorption spectroscopy part II: time resolved methods 5. Linear and circular dichroism 6. Fluorescence I: introduction to the method, excitation and emission spectra, fluorophores, FRET 7. Fluorescence II: time resolved fluorescence, fluorescence correlation spectroscopy 8. Optical microscopy: confocal microscopy, localization, superresolution methods 9. Rotational-vibrational spectroscopy: introduction 10. FTIR 11. Methods of Raman spectroscopy, resonance Raman spectroscopy 12. Theoretical practicals to summarise covered topics, example solution of selected problems by using optical spectroscopy methods Content of practicals: In practicals, the information from the lectures will be covered in more detail, methods will be demonstrated.

Learning activities and teaching methods
Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Demonstration, Excursion
  • Preparation for classes - 40 hours per semester
  • Class attendance - 40 hours per semester
Learning outcomes
The course will present theoretical and practical information on optical experimental methods and their use in biochemistry
The students will acquire understanding of the theoretical background of absorption and emission of light. The course covers principles, advantages, disadvantages and important experimental aspects of covered methods of optical spectroscopy and imaging. A large part of the covered methods will be demonstrated on biochemically relevant samples.
The students should understand introductory topics of light as electromagnetic radiation and its properties. The desired level is about that of advanced high school physics curriculum.

Assessment methods and criteria
Combined exam

Correct answers to at least 50% of exam questions.
Recommended literature
  • Lakowicz J.R.: Principles of fluorescence spectroscopy, 3rd edition, Springer, 2006.
  • Prosser V., ed.: Experimentální metody biofyziky, Academia, 1989.
  • Selvin P.R., Ha T.: Single-molecule techniques, CSH Press, New York, 2008.
  • van Holde K.E., Johnson W.C., Ho P.S.: Principles of Physical Biochemistry, 2nd edition, Prentice Hall, Upper Saddle River, NJ, 2005.

Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester