Course: Electron Microscopy II

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Course title Electron Microscopy II
Course code UFY/EM2
Organizational form of instruction Lecture + Lesson
Level of course Master
Year of study not specified
Frequency of the course In each academic year, in the summer semester.
Semester Summer
Number of ECTS credits 4
Language of instruction English
Status of course Compulsory-optional
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Course availability The course is available to visiting students
Lecturer(s)
  • Nebesářová Jana, Ing. CSc.
  • Kaftan David, Mgr. Ph.D.
  • Gardian Zdenko, Ing. Ph.D.
  • Bílý Tomáš, Mgr. Ph.D.
Course content
Lectures: The visualisation of biological macromolecules in high resolution. Qualitative and quantitative analysis of biological objects in electron microscopes. Progressive methods of biological specimen preparation. Advanced methods of immunolabeling. 3D electron microscopy. Electron tomography: from sample preparation to 3D visualization of CLEM = correlative light and electron microscopy. Probe microscopies (AFM, STM) and their biology applications. Practical: 3D reconstruction of the biological specimen using the method of electron tomography Single particle analysis of negatively stained samples Cryo-cutting and immunolabeling Demonstration of AFM

Learning activities and teaching methods
Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Laboratory
  • Preparation for credit - 24 hours per semester
  • Preparation for classes - 28 hours per semester
  • Preparation for exam - 24 hours per semester
  • Class attendance - 24 hours per semester
Learning outcomes
Lectures: Immunoelectron microscopy with unlabelled and labeled antibodies and their applications. Cryometries in Biological Electron Microscopy. Electronic tomography. Visualization of biological macromolecules. Autoradiography. Image analysis, three-dimensional reconstruction in electron microscopy. Morphometry and stereology. Electronic microanalysis (quality, quantity) of biological objects. Correlative microscopy. Electronic diffraction and its application in biology. New microscopy (STM, AFM) and their biological applications. A Lab exercise: Preparation of ultra-thin sections, sectional immunoassay, identification of crystalline structure by electron diffraction, determination of particle size in suspension by means of SEM, processing of measurement in ACC program. Překladač Google pro firmy:Translator ToolkitPřekladač webových stránek
Students receive an overview of advanced methods of electron and probe microscopy and knowledge of their use in biology and biolmedical research. Students acquire basic knowledge about the preparation of samples for those advanced methods. Students learn about cellular ultra-structure, and get an idea of the the possibilty to detect biomolecules. Students learn about the possibility of using electron microscopes for quantitative and qualitative electron microanalysis.
Prerequisites
Exam from the course "Electron Microscopy I"

Assessment methods and criteria
Test

Recommended literature
  • MJ Dykstra, LE Reuss: Biological Electron Microscopy ? Theory, Techniques and Troubleshooting. 2nd Edition..
  • Jana Nebesářová. Elektronová mikroskopie pro biology. Č.Budějovice, 2002.
  • John Kuo. Electron Microscopy. Springer Heidelberg, 2014. ISBN 978-1-62703-776-1.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Science Study plan (Version): Biological chemistry (1) Category: Chemistry courses - Recommended year of study:-, Recommended semester: Summer