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Lecturer(s)
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Polívka Tomáš, prof. RNDr. Ph.D.
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Course content
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1) Light-matter interaction: basic principles, energy levels, allowed vs. forbidden transitions, selection rules, dipole moment, Franck-Condon factors 2) Light sources and detectors: characteristic of light -intensity, polarization, photon energy, coherence; 'standard' sources vs. lasers; types of lasers - cw vs. pulses 3) Absorption spectroscopy: Lambert-Beer law, quantities characterizing absorption spectra, principles of measurements, scattering samples) 4) Fluorescence spectroscopy: Einstein coefficients, Stokes shift, mirror symmetry of absorption and fluorescence spectra, Strickler-Berg relation, fluorescence anisotropy, fluorescence excitation spectra 5) Circular Dichroism: magnetic transition dipoles, origin of circular dichroism, CD spectra of excitonic pair, CD spectra of proteins and nucelic acids, notes about linear dichroism 6) Raman Spectroscopy: 7) Basic time-resolved absorption methods: Pump-probe, Flash photolysis, different detection modes, chirp, multi-pulse variants of pump-probe spectroscopy 8) Time resolved fluorescence methods: Fluorescence up-conversion, streak camera and single-photon counting 9) Time resolved vibrational methods - femtosecond Raman and infrared spectroscopy 10) Multidimensional methods - transient grating, photon echo, 2D electronic spectroscopy 11) Analysis of time resolved data: global fitting methods, evolution associated spectra, target analysis
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Work with text (with textbook, with book), Demonstration, Work with multi-media resources (texts, internet, IT technologies)
- Class attendance
- 40 hours per semester
- Preparation for classes
- 40 hours per semester
- Field trip
- 4 hours per semester
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Learning outcomes
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The course will provide basic overview of spectroscopic methods commonly used in biophysics. Students will get theoretical basis of each methods, but the focus will be mainly on specific examples of using these methods in biophysics.
Students will gain knowledge about basic spectroscopic methods commonly applied in biophysics. The course is focused mainly on practical applications of the methods for solving problems in biophysics.
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Prerequisites
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unspecified
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Assessment methods and criteria
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Oral examination, Student performance assessment, Interview, Colloquium
To pass the course, students must solve given biophysical problem - students select suitable method and propose experimental solution of the problem.
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Recommended literature
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J. M. Hollas. Modern Spectroscopy, Wiley 2010.
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P. J. Walla. Modern Biophysical Chemistry, Wiley 2014.
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Wiliam W. Parson. Modern Optical Spectroscopy, Springer 2009.
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