Course: Immunological Laboratory Exercises

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Course title Immunological Laboratory Exercises
Course code ULZ/HHILC
Organizational form of instruction Lecture + Lesson
Level of course Bachelor
Year of study 3
Semester Winter
Number of ECTS credits 6
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Tinavská Pavlína, RNDr. Ph.D.
Course content
Lectures 1. Premunition 2. Immunopathological humoral reactions 3. Immunopathological reactions mediated by cells 4. Autoimmunity immunopathological conditions, inductions, effector mechanisms 5. Primary immunodeficiency 6. Secondary immunodeficiency 7. AIDS 8. CVID 9. Tumour immunity. 10. Transplantation. 11. Modulation of immunity system 12. Presentation of own works. Laboratory exercises 1. Familiarization with laboratory and organization of laboratory work 2. Preparation of ELISA tests 3. Preparation of fluorescent preparations 4. Work with fluorescent microscope 5. Foundations of work with through-flow cytometer 6. Diagnostics of infectious diseases with the help of through-flow cytometer 7. Diagnostics of blood malignities on through-flow cytometer 8. Diagnostics of auto-antibodies 9. Interpretation of positive findings of system auto-antibodies 10. Interpretation of positive findings of tissue-specific auto-antibodies 11. Interpretation of results of humoral immunity 12. Interpretation of results of cellular immunity

Learning activities and teaching methods
Learning outcomes
The student gets grasp of immunopathological conditions and of displays of immunopathological conditions in parameters of humoral and cellular immunity.
Students will have an overview of immunopathological conditions and how they are reflected in the parameters of immunologic laboratory tests.
Conditioning subjects: ULZ/HIMUL (KLI/HIMUL), ULZ/HMIK2 Student should manage bases haematology and microbiology.

Assessment methods and criteria
Oral examination

Requirements for granting the credit: pass examination tests, achieving minimally 14 points (maximally 20 points) maximally one absence with leave from exercises Requirements for granting the exam: The exam has oral form, it includes a question from general and clinical immunology and a question related to laboratory examination methods
Recommended literature
  • BARTŮŇKOVÁ J. Imunodeficience. Grada Praha, 2002. ISBN 80-247-0244-4.
  • BARTŮŇKOVÁ J., POULÍK M. Vyšetřovací metody v imunologii. Grada Praha, 2011. ISBN 978-80-247-3533-7.
  • HOŘEJŠÍ I., BARTŮŇKOVÁ J. Základy imunologie. Triton, 2009. ISBN 978-80-7387-280-9.
  • KOPECKÝ O. Běžná variabilní imonodeficience. Nucleus HK, 2006. ISBN 80-86225-93-3.
  • KREJSEK J., KOPECKÝ O. Klinická imunologie. Nucleus HK, 2004. ISBN 80-86225-50-X.

Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Health and Social Sciences Study plan (Version): - (2) Category: Health service 3 Recommended year of study:3, Recommended semester: Winter