Course: Clinical Biochemistry and Instrumental Analysis 2

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Course title Clinical Biochemistry and Instrumental Analysis 2
Course code ULZ/HHKB2
Organizational form of instruction Lecture
Level of course Bachelor
Year of study 3
Semester Winter
Number of ECTS credits 3
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
  • Racek Jaroslav, prof. MUDr. DrSc.
  • Verner Miroslav, MUDr.
Course content
Lectures: First Clinical biochemistry and its position between laboratory disciplines. Second Properties of laboratory methods in terms of analytical and clinical. Sensitivity (sensitivity), specificity, efficiency test, the relationship between sensitivity and specificity, some other indicators for assessing laboratory test. Third Energy, intermediary metabolism. Metabolic and energy balance. Oxygen metabolism in the body. Internal environment, anabolic and catabolic reactions of the organism. Metabolism of water, sodium, potassium and chloride, balance tracking. Osmolality. 4th Analytical systems employed for the analysis of the internal environment in clinical laboratories and at the bedside 5th Acid-base balance and its disorders, blood gases. Metabolism of calcium, magnesium and phosphorus. 6th Basic functions of the kidneys and their disorders, glomerular filtration, tubular function, laboratory calculations. Non-protein nitrogen compounds - urea, creatinine, uric acid, ammonia, amino acids. Diuresis - a distinction between changes, acute and chronic renal failure, measuring the effectiveness of hemodialysis, hemofiltration and peritoneal dialysis, renal transplantation. 7th Biochemical analyzers, analytical systems in routine biochemistry. 8th Plasma proteins. The importance of plasma. proteins, individual blood plasma proteins, acute phase response. 9th Protein instrumental analysis. 10th Cardiovascular system. Risk factors for the development of atherosclerosis. 11th Laboratory diagnosis of ischemic heart disease - used laboratory procedures. 12th Laboratory tests for diabetes mellitus (DM). Instrumental diagnosis of DM, the determination of glucose and other appropriate analytes. Determination of autoantibodies, early diagnosis of complications, DM and pregnancy, other causes of hypo-and hypeglykémie. 13th Immunochemical analysis systems. 14th Laboratory tests for liver disease. Laboratory signs of hepatocyte damage, impaired function of hepatocytes, toxic liver damage, cholestasy laboratory evidence, laboratory evidence of hepatic fibrosis, typical laboratory findings in some liver diseases. Differential diagnosis of hyperbilirubinemia. 15th Laboratory signs of malignant neoplasm. 16th Clinical and biochemical examination of the gastrointestinal tract. Enetrální and parenteral nutrition in terms of clinical biochemistry. Investigation and monitoring of nutritional status. Malnutrition. Obesity. 17th Laboratory tests in pregnancy. Adaptive response of the organism to pregnancy, changes in physiological range of laboratory tests in pregnancy. Systems used for diagnostics. Peculiarities of laboratory testing in childhood and in old age. 18th Examination of the cerebrospinal fluid, used laboratory procedures. 19th Hormones. General knowledge of the metabolism and determination of hormones, hormones, hypothalamus, pituitary and pineal gland, thyroid hormones, adrenal hormones, gonads and sex hormones, tissue hormones. Special analyzers. 20th Trace elements. Iron (Fe), copper (Cu), zinc (Zn), selenium (Se) and other trace elements. Vitamins. 21st Laboratory diagnosis in intensive care. Reference values ??of laboratory tests. Definitions, basic concepts, methods of determining the reference range, the method of measurement values ??compared with the reference values, practical notes for determination of reference limits and evaluation of results 22nd Quality control laboratory testing.

Learning activities and teaching methods
Monologic (reading, lecture, briefing)
Learning outcomes
Sylabus předmětu obsahuje teorii biochemických aspektů, fyziologických a patofyziologických pochodů v organizmu, součástí každé kapitoly jsou též odkazy na principy příslušných laboratorních metod, které budou později prakticky cvičeny v kurzu Klinická biochemie - laboratorní cvičení.
Course syllabus includes the theory of biochemical aspects of physiological and pathophysiological processes in the body, a part of each chapter are also relevant principles of laboratory methods.
Conditioning subjects: ULZ/HHKB1

Assessment methods and criteria

Requirements for granting the credit: taching activity, test Requirements for granting the exam: exam Student has passed the test of Biochemistry, teaching the subject to which Clinical Biochemistry and instrumental analysis follows .
Recommended literature
  • DASTYCH, BREINEK a kol.:. Klinická biochemie, MU lékařská fakulta, Brno 2008.
  • RACEK et al.:. Klinická Biochemie, Galén, Praha 2006..
  • ŠTERN a kol.:. Obecná a klinická biochemie pro bakalářské obory studia, Karolinum 2005.
  • ZIMA, KAZDA, PRŮŠA et al. Laboratorní diagnostika, Galén, Praha 2007.

Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Health and Social Sciences Study plan (Version): - (2) Category: Health service 3 Recommended year of study:3, Recommended semester: Winter