Course: Clinical Biochemistry and Laboratory Exercises

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Course title Clinical Biochemistry and Laboratory Exercises
Course code ULZ/HHKBC
Organizational form of instruction Lesson
Level of course Bachelor
Year of study 3
Semester Winter
Number of ECTS credits 3
Language of instruction Czech
Status of course Compulsory
Form of instruction Face-to-face
Work placements This is not an internship
Recommended optional programme components None
Lecturer(s)
  • Kašparová Marie, Ing.
Course content
1. First Good laboratory work, pipetting, weighing , preparation of solutions , centrifugation , basic biological samples , dilution of biological material , expressing analytical results ( SI) , information retrieval - Internet 2. Second Sampling of biological material, preanalytical factors, blood collection for determination of blood glucose 3. Third Physical and chemical examination of urine - diagnostic strips, chemical tests , reflex photometry 4. Urinary sediment native, dyed , SW sediment, sediment Hamburger , flow cytometry 5. Stool examination chemical and microscopic 6. Photometry, working with a light meter , determination of total protein , glucose , enzymes , validation of analytical methods - a protocol 7. Working with biochemical analyzer , turbidimetry , nephelometry, laboratory information system, internal quality control 8. Parameters of the internal environment - acid-base balance , ISE , flame atomic emission spectrophotometry, osmometry 9. Electrophoretic methods - serum protein electrophoresis , urine, hemoglobin, immunofixation electrophoresis of proteins, densitometry 10. Immunochemical methods ( LIA, ECLIA , FPIA , ELISA , RIA ) - cardiac , hormones , drugs , tumor markers 11. Examination of cerebrospinal fluid - the basic biochemistry , cytology - differentiation elements 12. Chromatography - TCL, HPTLC , HPLC , GC, GCMS (pharmacology and toxicology )

Learning activities and teaching methods
Laboratory
Learning outcomes
The course includes practical training in basic biochemical methods.
Students will acquire basic knowledge of proper laboratory work both in terms of laboratory instrumentation and in terms of analysis.
Prerequisites
Conditioning subjects: No

Assessment methods and criteria
Oral examination, Student performance assessment, Test

Requirements for granting the credit: 90% attendance at seminars, teaching activity, Active student participation in seminars, practical knowledges
Recommended literature
  • DASTYCH, BREINEK a kol. Klinická biochemie. MU lékařská fakulta, Brno, 2008. ISBN 80-210-2363-5.
  • CHROMÝ, FISCHER. Analytické metody v klinické chemii. MU přírodovědecká fakulta, Brno, 2000. ISBN 80-210-2363-5.
  • MASOPUST Z. Požadování a hodnocení biochemických vyšetření I, II.. Karolinum, Praha, 1998. ISBN 80-7184-649-3.
  • RACEK et al. Klinická biochemie. Galén Praha, 2006. ISBN 80-7262-324-9.
  • ŠTERN a kol. Obecná a klinická biochemie pro bakalářské obory studia. Karolinum, 2005. ISBN 80-246-1025-6.
  • ZIMA, KAZDA, PRŮŠA et al. Laboratorní diagnostika. Galén, Praha, 2007. ISBN 978-80-7262-372-3.


Study plans that include the course
Faculty Study plan (Version) Category of Branch/Specialization Recommended year of study Recommended semester
Faculty: Faculty of Health and Social Sciences Study plan (Version): - (2) Category: Health service 3 Recommended year of study:3, Recommended semester: Winter