Lectures: 1. Cell theory. Living systems and system hierarchies. Methods of cell biology 2. The cell and its components, prokaryotic and eukaryotic cell. Supramolecular complexes and organelles 3. The cell and its surroundings. Cell surface: cell wall, intercellular junctions, plasma membrane. Cell-environment relations. Passive transport of small molecules. Active transport of small molecules, proton pump, sodium-potassium pump. Large molecule transfer, endocytosis and exocytosis, clathrin-coated wells and vesicles, caveoles, clathrin- and caveolin-free vesicles, macropinosomes, endosome recycling 4. Receipt and transmission of information. Signal reception and transmission, physical signals, light-induced motion, vision, external and internal chemical signals, second messenger services, receptors 5. Ribosomes, structure, proteosynthesis. Endoplasmic reticulum, structure and function, role in quality control of proteosynthesis. Golgi complex, structure, function, postsynthetic modifications of proteinsLysosomes, melanosomes, peroxisomes, tapetosomes. Vacuoles. Inclusion 6. Energy assurance of cell life. Mitochondria, structure and function. Plastids, structure and function, chloroplasts, thylakoid membrane 7. Cytoskeleton. Dynamic cytoplasmic matrix, molecular organization, interactions. Microtubules, MAPs, MTOC, basic types of microtubular motors. Microfilaments, muscle and non-muscle movement. Intermediate filaments, stability and structure. Binding of the cytoskeleton to membranes. Membrane and nuclear skeleton. Centrosome, centrioles, structure and microtubules. Whips. Mascara, ciliogenesis. Mechanisms of movement at the cellular and subcellular level 8. Cell nucleus. Chromatin, sexchromatin, chromosomes, nuclear membrane, nucleolus. Cell cycle. Mitosis, meiosis, replication, transcription 9. Multicellularity. Adhesion, adhesion molecules; intercellular connections and communications; intercellular mass, structure and function 10. Cell death. Programmed cell death and apoptosis, the role of cell death in the development of unicellular organisms, plants and animals, partial mechanisms. Cell necrosis Laboratory training: 1. Digital imaging methods in biology and medicine. Practical use of CCD camera. Fluorescence microscopy 2. Cytochemistry, histochemical detection of sugar components of the plasma membrane using lectin 3. Endoplasmic reticulum - Niss staining 4. Representation of the Golgi apparatus according to Elftman 5. Vital staining of mitochondria 6. Lysosomes and panoptic staining 7. Cytoskeleton: visualization of tubulin by iminofluorescence 8. Inclusions - detection of lipid inclusions of sudanophilia 9. Detection of the nucleus by pyrinin 10. Staining of nucleus structures containing RNA according to Smetana, nucleolus, Barr bodies of epithelia of female oral mucosa - detection by Lugoll's solution
|
-
Alberts B et al. Essential Cell Biology. Garland Sci, New York, 2009. ISBN 978-0-8153-4130-7.
-
Berger J. Biologie buněk. Kopp, České Budějovice, 2000.
-
HARDIN J. et al.:. Becker's World of the Cell. Benjamin Cummings, Boston, 2011. ISBN 978-0321716026.
-
MAILLET M. Biologie cellulaire. Masson, Paris, 2006. ISBN 78-2294019944.
-
PALEČEK J. Biologie buňky, Základy mikroskopické cytologie. Karolinum, Praha, 2000. ISBN 8071842664.
|