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Lecturer(s)
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Course content
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Lectures: 1. Intramolecular interactions in metabolites and macromolecules. 2. Intermolecular interactions in metabolites and macromolecules. 3. Radiation in instrumental analysis. 4. Electroanalytical methods. 5. Sample preparation for analysis, principles of extraction methods. 6. Principle of separation of substances, chromatography. 7. Principle of substance separation, electromigration methods, electrophoresis. 8. Immunochemical methods. 9. Mass spectrometry (MS) and nuclear magnetic resonance (NMR). 10. Individual phases of instrumental analysis and basics of evaluation of analytical measurement results. Seminars: 1. Intramolecular, intermolecular interactions, radiation and electroanalytical methods in instrumental analysis. (2 hours) 2. Principles of extraction, chromatography and electromigration methods. (2 hours) 3. Immunochemical methods. (2 hours) 4. Principles of mass spectrometry and NMR methods. (2 hours) 5. Phases of instrumental analysis and basics of evaluation of analytical measurement results. (2 hours)
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Learning activities and teaching methods
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unspecified
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Learning outcomes
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The course explains the principles of biological material analysis using instrumentation, and provides an opportunity to understand the logic of the importance of using various instruments for chemical and
1. The student understands the nature of intramolecular interactions of substances and intermolecular interactions between substances and the environment at the level of small molecules and macromolecular systems. 2. The student can describe the interactions between matter and radiation, the principle of absorption and emission of radiation, scattering, reflection and refraction of light and understand the principles of methods based on these interactions. 3. The student can describe the electrical cell, electrode potential, and understand the principles of pH measurement and the use of ion-selective electrodes in potentiometric measurements. 4. The student can describe the principles of sample preparation for analysis and separation of substances, extraction of substances from various materials, the principle of derivatization and chromatography in the liquid and gas phase, including graphic outputs, chromatograms. 5. The student can describe the principles of electromigration methods, especially electrophoresis and blotting and understands the main factors that affect these separation processes. 6. The student understands the principle of immunochemical methods and various arrangements of immunoanalytical methods, especially the ELISA method. 7. The student understands the basic principles of mass spectrometry and NMR, the principles of hard and soft ionization of substances, isotopic composition and can describe the basic characteristics of the mass spectrum and NMR spectrum 8. The student can describe the individual phases of laboratory analysis of substances, the principles of qualitative, quantitative analysis, measurement uncertainties, calibration and validation of analytical methods
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Prerequisites
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unspecified
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Assessment methods and criteria
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unspecified
Combined exam
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Recommended literature
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DOLEŽALOVÁ V. et al. Laboratorní technika v klinické biochemii a toxikologii. IDVPZ, Brno, 1995.
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FOGL J.:. Návody pro laboratorní cvičení z analytické chemie. I. VŠCHT Praha, 2000.
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Chromý V. et al:. Bioanalytika. Analytická chemie v laboratorní medicíně. MU, 2002. ISBN 80-210-2917-X.
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Skoog DA, Holler FJ, Crouch SR. Principles of Instrumental Analysis. , 6th edition. Brooks/Cole Pub Co, 2006. ISBN 978-0495012016.
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Šimek P. Základy instrumentální analýzy. Přednášky předmětu v pdf formátu. Č. Budějovice, 2012.
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Zima T. et al.:. Laboratorní diagnostika. Galen, 2007. ISBN 80-24614235.
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