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Lecturer(s)
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Grabicová Kateřina, Ing. Bc. Ph.D.
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Course content
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Subject "Biochemistry" includes following topics: 1) Subject of biochemistry, basic composition of organisms, biological function and structure of cell 2) Sugars: function and significance in the organism, monosaccharides, oligosaccharides and polysaccharides, binding of sugars to other macromolecules, lectins 3) Lipids and membranes, fatty acids, basic types of lipids, transport of substances across the membrane, membrane channels and pumps 4) Structure and function of proteins, amino acids as the basic components of proteins, nucleic acids (DNA, RNA) - composition: sugars, type of bases, formation of the chain; structure: double-helix, complemetarity of bases 5) Enzymes: basic characteristics, thermodynamics of enzyme catalysis, kinetics of the enzyme catalysis: model of Michaelis-Menten, enzyme inhibition 6) Principles of cellular metabolism, macroergic bonds, ATP as the universal source of energy for biosynthetic reactions, regulation of metabolism 7) Metabolism of sugars - glycolysis, glukoneogenesis, metabolism of glycogen; metabolism of lipids and fatty acids 8) Metabolism of proteins, amino acids and nucleotides 9) Cycle of tricarboxylic acid, respiration and oxidative phosphorylation 10) Gene expression: transformation of the genetic information into the functional molecule (transcription, translation); proteosynthesis 11) Regulation and integration of metabolism - hormones, neurotransmitters 12) Defence mechanisms of organism - immune system, metabolism of xenobiotics 13) Photosynthesis - light reactions of photosynthesis, dark (Calvin cycle) a pentose cycle
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Learning activities and teaching methods
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Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming)
- Preparation for exam
- 30 hours per semester
- Preparation for credit
- 20 hours per semester
- Class attendance
- 10 hours per semester
- Preparation for classes
- 72 hours per semester
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Learning outcomes
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The aim of the subject is to give students basic knowledge about general and structural biochemistry. Students will learn about the principal components of living organisms and their role in the biological processes at the molecular level. The practical training will be focused on nomenclature, structure of molecules and calculation in enzymology.
The graduate students will get basic knowledge about the structure and function of the principal components of living organisms and their transformations at the molecular level.
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Prerequisites
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Students should pass the subjects Chemistry 1 and 2.
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Assessment methods and criteria
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Oral examination, Test
Conditions for obtaining credit: 80% attendance of training lessons. Students have to pass 4 credit tests (obtaining more than 50 % points in all tests). Conditions for oral examination: The examination will be only oral.
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Recommended literature
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Kodíček, M. Biochemické pojmy -výkladový slovník. VŠCHT Praha, 2004.
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Kodíček, M., Valentová, O., Hynek, R. Biochemie - chemický pohled na biologický svět. Praha, 2015. ISBN 978-80-7080-927-3.
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Vodrážka, Z. Biochemie. Academia Praha, 2007.
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